纤维素酶辅助超声提取丁香叶黄酮工艺优化及抗氧化性分析  

Optimization of Cellulase-Assisted Ultrasound Extraction and Antioxidant Analysis of Flavonoids from Syringa oblata Leaves

作　　者：黄磊磊 刘佳怡 王天怡 张庆芬 杨逢建 HUANG Leilei;LIU Jiayi;WANG Tianyi;ZHANG Qingfen;YANG Fengjian(Key Laboratory of Forest Plant Ecology of the Ministry of Education,Northeast Forestry University,Harbin 150040,China;School of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry,Harbin 150040,China;Engineering Center of the Ministry of Forestry Biologics Education,Northeast Forestry University,Harbin 150040,China;Key Laboratory of Ecological Utilization of Forest Source Active Substances in Heilongjiang Province,Harbin 150040,China;National and Local Joint Engineering Laboratory for Ecological Utilization of Biological Resources,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学,森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [3]东北林业大学林业生物制剂教育部工程中心,黑龙江哈尔滨150040 [4]黑龙江省林源活性物质生态利用重点实验室,黑龙江哈尔滨150040 [5]东北林业大学生物资源生态利用国家地方联合工程实验室,黑龙江哈尔滨150040

出　　处：《食品工业科技》2024年第4期161-170,共10页Science and Technology of Food Industry

基　　金：黑龙江省林业科技项目(Hljlykj2018-1)。

摘　　要：为研究丁香叶中总黄酮的高效提取方法,本文首次采用纤维素酶辅助超声法对4种丁香叶中的总黄酮进行提取。以总黄酮提取得率为指标,单因素实验分析纤维素酶用量、pH、超声时间、超声功率、液料比、温度、乙醇体积分数对小叶丁香叶总黄酮提取得率的影响,应用响应面法优化,得到最优提取工艺,并通过体外抗氧化检测丁香叶总黄酮对DPPH自由基和OH自由基的清除能力。结果表明,最优提取工艺为加酶量2%,pH5.5,超声时间40 min,超声功率400 W,乙醇体积分数63%,温度56℃,液料比30:1(mL/g),在该条件下,小叶丁香叶总黄酮提取得率为32.21±0.16 mg/g,与回归模型的预测值32.57 mg/g接近。抗氧化活性结果表明,4种丁香叶总黄酮对DPPH自由基的清除能力为小叶丁香>暴马丁香>白丁香>紫丁香;对OH自由基的清除能力为小叶丁香>暴马丁香>紫丁香>白丁香。纤维素酶辅助超声法能高效提取丁香叶中的总黄酮,且丁香叶总黄酮具有较好的抗氧化性,具有一定的开发潜力。To investigate the efficient method of total flavonoids from Syringa oblata leaves,this paper firstly used cellulase-assisted ultrasound method to extract flavonoids from four Syringa oblata leaves.The effects of cellulase dosage,pH,ultrasound time,ultrasound power,liquid-material ratio,temperature and ethanol volume fraction on the extraction rate of total flavonoids from Syringa pubescens Turcz.leaves were analyzed by single-factor experiments,and the best extraction process was obtained by applying response surface methodology optimization.The scavenging ability of Syringa oblata leaves flavonoids on DPPH and OH radicals was also examined in vitro as an antioxidant.The results showed that,the optimum extraction process was enzyme addition 2%,pH5.5,sonication time 40 min,sonication power 400 W,ethanol volume fraction 63%,temperature 56℃,liquid to material ratio 30:1(mL/g),and under these conditions,the Syringa oblata flavonoids content was 32.21±0.16 mg/g,which was in proximity to the predicted value of 32.57 mg/g from the regression model.The results of antioxidant activity showed that the scavenging ability of the four Syringa oblata leaf flavonoids on DPPH was Syringa pubescens Turcz.>Syringa reticulata var.mamds huricq.>Syringa oblata var.affinis lingdelsh.>Syringa oblata Linal.The scavenging ability on OH was Syringa pubescens Turcz.>Syringa reticulata var.mamds huricq.>Syringa oblata Linal.>Syringa oblata var.affinis lingdelsh.The cellulase-assisted ultrasonication method can efficiently extract flavonoids from Syringa oblata leaves,and the flavonoids of Syringa oblata leaves have good antioxidant properties and have certain potential for development.

关 键 词：丁香叶 纤维素酶辅助超声提取 总黄酮 响应面法 抗氧化性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]