非小细胞肺癌组织miR-1179、miR-1182表达水平与Notch信号通路、临床病理特征和预后的关系  

Relationship between miR-1179 and miR-1182 Expression Levels in Non-Small Cell Lung Cancer Tissues and Notch Signaling Pathway,Clinicopathological Features and Prognosis

作　　者：童亚男[1] 雷俊[1] 田辉[1] 郑洋 明冠 何威华[1] TONG Ya-nan;LEI Jun;TIAN Hui;ZHENG Yang;MING Guan;HE Wei-hua(Department of Pulmonary Diseases,Wuhan Traditional Chinese Medicine Hospital,Wuhan,Hubei,430000,China)

机构地区：[1]武汉市中医医院肺系病科,湖北武汉430000

出　　处：《现代生物医学进展》2023年第22期4369-4374,共6页Progress in Modern Biomedicine

基　　金：湖北省中医药管理局中医药科研项目(ZY2023F054);武汉市医学科研项目中医类(WZ21Q19)。

摘　　要：目的:探讨非小细胞肺癌(NSCLC)组织微小核糖核酸(miRNA)-1179、miR-1182表达与缺口(Notch)信号通路、临床病理特征和预后的关系。方法:选取2018年1月~2019年12月武汉市中医医院收治的118例NSCLC患者,收集手术切除的癌组织和癌旁组织标本,采用实时荧光定量聚合酶链式反应检测miR-1179、miR-1182和Notch信号通路相关分子表达。分析miR-1179、miR-1182表达与Notch信号通路相关分子和NSCLC患者临床病理特征的关系。根据NSCLC组织中miR-1179、miR-1182表达均值分为高、低表达组,采用K-M法绘制不同miR-1179、miR-1182表达NSCLC患者生存曲线,多因素Cox回归分析NSCLC患者预后的影响因素。结果:与癌旁组织比较,NSCLC组织中miR-1179、miR-1182表达降低,Notch受体1(Notch1)信使核糖核酸(mRNA)、Notch2 mRNA、Notch3 mRNA、Notch4 mRNA表达升高(P<0.05)。Pearson相关性分析显示,NSCLC组织中miR-1179、miR-1182表达与Notch1 mRNA、Notch2 mRNA、Notch3 mRNA、Notch4 mRNA表达均呈负相关(P<0.05)。不同分化程度、TNM分期、淋巴结转移NSCLC患者miR-1179、miR-1182表达比较有统计学差异(P<0.05)。118例NSCLC患者随访3年,失访5例,3年总生存率为55.75%。K-M生存曲线分析显示,miR-1179、miR-1182高表达组总生存率高于低表达组(P<0.05)。多因素Cox回归分析显示,低分化、TNM分期Ⅲ期、淋巴结转移为NSCLC患者预后的独立危险因素,miR-1179、miR-1182升高为其独立保护因素(P<0.05)。结论:NSCLC组织中miR-1179、miR-1182低表达,与Notch信号通路、分化程度、TNM分期、淋巴结转移和预后有关,miR-1179、miR-1182表达可能通过抑制Notch信号通路发挥抑癌作用。Objective:To investigate the relationship between tissue microRNA(miRNA)-1179 and miR-1182 expression and Notch signaling pathway,clinicopathological features and prognosis in non-small cell lung cancer(NSCLC).Methods:118 cases of NSCLC patients admitted to Wuhan Traditional Chinese Medicine Hospital from January 2018 to January 2019 were selected,and some intraoperative cancer tissues and paracancerous tissues were collected,and miR-1179,miR-1182 and Notch signaling pathway-related molecules expression were detected by real-time fluorescence quantitative polymerase chain reaction.To analyze the relationship between miR-1179 and miR-1182 expression and Notch signaling pathway-related molecules and clinicopathological features of NSCLC patients.The mean values of miR-1179 and miR-1182 expression in NSCLC tissues were divided into high and low expression groups,and the survival curves of NSCLC patients with different miR-1179 and miR-1182 expression were plotted using the K-M method,and the factors influencing the prognosis of NSCLC patients were analyzed using multi-factor Cox regression.Results:Compared with paraneoplastic tissues,miR-1179 and miR-1182 expression was decreased and Notch receptor 1(Notch1)mRNA,Notch2 mRNA,Notch3 mRNA,and Notch4 mRNA expression was increased in NSCLC tissues(P<0.05).Pearson correlation analysis showed that miR-1179 and miR-1182 expression in NSCLC tissues were negatively correlated with Notch1 mRNA,Notch2 mRNA,Notch3 mRNA,and Notch4 mRNA expression(P<0.05).There were differences in miR-1179 and miR-1182 expression in NSCLC patients with different degrees of differentiation,TNM stage,and lymph node metastasis(P<0.05).The 3-year overall survival rate of the 118 NSCLC patients was 55.75%.K-M survival curve analysis showed that the overall survival rate of miR-1179 and miR-1182 high expression group was higher than that of low expression group(P<0.05).Multivariate Cox regression analysis showed that hypofractionation,TNM stage III,and lymph node metastasis were independent risk factors

关 键 词：非小细胞肺癌 miR-1179 miR-1182 NOTCH信号通路 临床病理特征 预后 

分 类 号：R734.2[医药卫生—肿瘤]