植物叶片悬浮培养的研究  

Research on Isotonic Suspensions of Plant Leaves

作　　者：安惠韬 王茜[1] 王瑞刚[1] AN Huitao;WANG Qian;WANG Ruigang(Inner Mongolia Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions,Inner Mongolia Hohhot 010018)

机构地区：[1]内蒙古农业大学旱寒区植物逆境适应与遗传修饰改良自治区重点实验室,内蒙古呼和浩特010018

出　　处：《林业科技》2024年第1期29-33,共5页Forestry Science & Technology

基　　金：呼和浩特市重大科技专项(2022-社-重-1-3);科技创新团队(NMGIRT2222和BR22-13-05)。

摘　　要：对于植物为适应胁迫环境的生理变化及其适应胁迫环境能力的研究是近年来的热点问题。渗透调节机制是植物适应胁迫环境的主要生理机制,其中植物叶片细胞渗透势的测定与研究是确定植物渗透调节能力的关键。本研究以拟南芥、柠条、落叶松为研究对象,经过梯度浓度蔗糖溶液培养,以质壁分离现象的发生为标准测定植物叶片细胞渗透势。通过显微镜观察发现,拟南芥、柠条、落叶松叶片细胞等渗浓度分别为0.375、0.575、0.825 mol/L(蔗糖溶液)。计算得出,拟南芥、柠条、落叶松叶片细胞渗透势分别为-921.3、-1 412.66、-2 026.86 kPa。对南芥、柠条、落叶松等植物叶片细胞渗透势的测定,有利于提高植物组织培养效率、提高植物植物存活率。同时,为植物渗透调节机制的研究、优良品种的选育提供理论依据。Research on the physiological changes in plants to adapt to stressful environments and their ability to adapt to stressful environments has been a hot issue in recent years.Osmoregulation is the main physiological mechanism for plants to adapt to stressful environments,among which the determination and study of the osmotic potential of plant leaves is the key to determine the osmoregulatory capacity of plants.In this study,the osmotic potential of plant leaves was determined by the occurrence of the phenomenon of plasmatic wall separation after incubation with a gradient concentration of sucrose solution using arabidopsis,lemongrass and larch as the subjects.The isotonic concentrations of Arabidopsis thaliana,Caragana microphylla and Larix gmelinii were 0.375,0.575 mol/L and 0.825 mol/L(sucrose solution)respectively.The calculated cellular osmotic potential was-921.3,-1412.66,and-2026.86 kPa for Arabidopsis,lemongrass,and Larix gmelinii,respectively.The determination of the osmotic potentials of the leaves of Arabidopsis thaliana,Caragana korshinskii microphylla and Larix gmelinii was useful for improving the efficiency of plant tissue culture and plant survival.At the same time,it provides theoretical basis for the study of osmoregulation mechanism of plants and the selection and breeding of excellent varieties.

关 键 词：拟南芥 柠条 落叶松 质壁分离 渗透势 等渗 

分 类 号：S512.1[农业科学—作物学] Q95[生物学—动物学] Q71