蓝靛果原浆对果蝇寿命及其体内抗氧化活性的影响  

Effects of Lonicera eclulis pulp on lifespan and antioxidant activity in vivo of Drosophila melanogaster

作　　者：屈美华 张彦龙 李元敬[3] 曾伟民 孟鑫 雷虹[1,2] QU Meihua;ZHANG Yanlong;LI Yuanjing;ZENG Weimin;MENG Xin;LEI Hong(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China;Key Laboratory of Molecular Biology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080,China;College of Ocean and Environmental Engineering,Shanwei Campus of South China Normal University,Shanwei 516600,China)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080 [3]华南师范大学汕尾校区海洋与环境工程学院,汕尾516600

出　　处：《黑龙江大学自然科学学报》2023年第6期713-720,共8页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省自然科学基金(C2015023);“三区”科技人才支持计划项目(39011150105)。

摘　　要：以果蝇为模式生物,探究了蓝靛果原浆的抗衰老作用。果蝇按雌雄分组后,随机分为空白对照组以及低、中、高剂量组进行生存实验,并测定果蝇体内的抗氧化酶活力及丙二醛含量。与对照组相比,中、高剂量组雄果蝇的平均最长寿命分别显著延长了9.83%(P<0.05)、12.56%(P<0.05),中、高剂量组雌果蝇的平均最长寿命分别显著延长了16.92%(P<0.05)、17.41%(P<0.05)。抗氧化实验结果显示,饲喂蓝靛果原浆能够显著提高雌雄果蝇体内的抗氧化酶活力,降低丙二醛含量,且存在一定的剂量依赖性。实验结果表明,蓝靛果原浆具有抗衰老作用。The anti-aging effect of Lonicera eclulis pulp was studied,using Drosophila melanogaster as model organism.The male and female Drosophila melanogaster were randomly divided into blank control group and high,medium and low dose groups for survival experiment and the determination of antioxidant enzyme activity and malondialdehyde(MDA)content in Drosophila melanogaster.Compared with the control group,the average maximum lifespan of male drosophila was significantly prolonged in the medium-dose and high-dose groups,respectively 9.83%(P<0.05)and 12.56%(P<0.05)and the average maximum lifespan of female drosophila was significantly prolonged in the medium-dose and high-dose groups,respectively 16.92%(P<0.05)and 17.41%(P<0.05).The results of antioxidant experiment showed that feeding Lonicera eclulis pulp could significantly improve the activity of antioxidant enzymes and reduce the content of MDA in Drosophila melanogaster in a dose-dependent manner.The above results showed that Lonicera eclulis pulp had anti-aging effect.

关 键 词：抗衰老 果蝇 蓝靛果 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]