米槁叶绿体基因组结构及系统发育关系分析  

Analysis on Chloroplast Genome Characteristics and Phylogeny of Cinnamomum migao H.W.Li

作　　者：黎前利 吴媛 吴之坤 LI Qianli;WU Yuan;WU Zhikun(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵阳550025

出　　处：《种子》2023年第11期1-8,共8页Seed

基　　金：国家自然科学基金委员会-贵州省人民政府联合基金项目(U1812403-2);贵州省科技计划项目(黔科中引地[2022]4016)。

摘　　要：本研究利用Illumina全基因组测序数据组装了米槁叶绿体全基因组:基因组总大小为152751 bp,总GC含量为39.1%,包含一个93662 bp的大单拷贝区(LSC)、一个18861 bp的小单拷贝区(SSC)和一对20114 bp的反向重复区(IRs)。整个叶绿体基因组共注释了125个基因[81个编码基因(CDS)、8个rRNA基因和36个tRNA基因],包括14个多拷贝基因和96个单拷贝基因。系统进化分析表明,用以构建系统发育树的樟属植物并不聚类为一个单系支系;从不同地区采集的两个米槁样品形成了一个单系支系,支持率为100%,与其他樟属植物有明显差异。完整的米槁叶绿体基因组为米槁的系统发育、保护、分子鉴定和可持续利用提供了基础信息。In this study,the complete chloroplast genome of Cinnamomum migao was assembled using the whole genome Illumina sequencing data:the total genome size was 152751 bp with an overall GC content of 39.1%,containing a large single-copy(LSC)region of 93662 bp,a small single-copy(SSC)region of 18861 bp,and a pair of inverted repeats(IRs)regions of 20114 bp.In total,125 genes(81 coding genes(CDS),eight RNA genes,and 36 tRNA genes)were annotated in the whole cp genome,including 14 multicopy genes and 96 single-copy genes.The phylogenetic analysis indicated that the sampled Cinnamomum species did not cluster as a monophyletic clade.The two samples of C.migao collected from different areas formed a monophyletic clade with 100%bootstrap value,and clearly differed from other Cinnamomum species.The complete chloroplast genome of C.migao provide genomic iinformation necessary for the phylogeny,conservation,molecular identification and sustainable utilization of this plant.

关 键 词：米槁 樟科 叶绿体基因组 系统发育分析 

分 类 号：S567.19[农业科学—中草药栽培]