食管癌组织DPP3、SMAC表达变化及其与临床病理特征和预后的关系  

Expression changes of DPP3 and SMAC in esophageal carcinoma and their relationships with clinicopathological features and prognosis

作　　者：朱强 孙彦龙 武毅 王海 王玉璇 ZHU Qiang;SUN Yanlong;WU Yi;WANG Hai;WANG Yuxuan(Department of Thoracic Surgery,Shanxi Bethune Hospital,Taiyuan 030032,China)

机构地区：[1]山西白求恩医院(山西医学科学院同济山西医院)山西医科大学第三医院胸外科,太原030032 [2]华中科技大学同济医学院附属同济医院胸外科

出　　处：《山东医药》2024年第1期16-19,29,共5页Shandong Medical Journal

基　　金：山西省卫生健康科研课题(2019086)。

摘　　要：目的探讨食管癌组织二肽基肽酶Ⅲ(DPP3)、第二个线粒体衍生的半胱氨酸蛋白酶激活剂(SMAC)表达变化及其与临床病理特征和预后的关系。方法选择101例食管癌患者,均接受根治性或姑息性手术治疗,留取术中切除的食管癌组织及其癌旁正常组织,采用免疫组化法检测DPP3、SMAC阳性表达率。分析食管癌组织DPP3、SMAC阳性表达与临床病理特征的关系。出院后随访3年,统计食管癌患者生存情况,比较DPP3、SMAC阳性表达者与阴性表达者3年生存率。结果与癌旁正常组织比较,食管癌组织DPP3阳性表达率升高,SMAC阳性表达率降低(χ^(2)分别为49.744、32.020,P均<0.05)。食管癌组织DPP3、SMAC阳性表达与组织分化程度、TNM分期、淋巴结转移有关(P均<0.05)。随访3年,无失访病例,期间死亡33例,总生存率为67.33%(68/101)。生存分析显示,DPP3阳性表达者3年生存率低于其阴性表达者,SMAC阳性表达者3年生存率高于其阴性表达者(Log-rankχ^(2)分别为6.342、10.811,P均<0.05)。结论食管癌组织DPP3高表达、SMAC低表达,二者表达变化与组织分化程度、TNM分期、淋巴结转移和预后不良密切相关。Objective To investigate the expression changes of dipeptidyl peptidaseⅢ(DPP3)and second mitochondria-derived activator of caspase(SMAC)in esophageal carcinoma tissues and their relationships with clinicopathological features and prognosis.Methods Totally 101 esophageal cancer patients were selected,and intraoperatively resected esophageal cancer tissues and their adjacent normal tissues were detected by immunohistochemistry to compare the positive expression rates of DPP3 and SMAC in different tissues.We analyzed the relationships between the positive expression of DPP3 and SMAC in esophageal cancer tissues and clinicopathological features.After 3 years of follow-up after discharge,Kaplan-Meier curves were plotted to compare the 3-year survival rates of those with positive/negative expression of DPP3 and SMAC.Results Compared with the adjacent normal tissues,the positive expression rate of DPP3 in the esophageal cancer tissues increased,while the positive expression rate of SMAC decreased(χ^(2)=49.744,32.020,respectively;both P<0.05).The positive expression rates of DPP3 and SMAC in the esophageal cancer tissues were related to degree of tissue differentiation,TNM stage,and lymph node metastasis(all P<0.05).There were no lost cases during 3 years of follow-up and 33 deaths during this period,with the overall survival rate of 67.33%(68/101).Survival analysis showed that the 3-year survival rate was lower in DPP3-positive patients than in DPP3-negative ones,and was higher in SMAC-positive patients than in SMAC-negative ones(Log-rankχ^(2)=6.342,10.811,respectively;both P<0.05).Conclusion DPP3 is highly expressed in esophageal cancer tissues,while SMAC is lowly expressed,both of which are associated with the degree of tissue differentiation,TNM staging,lymph node metastasis and poor prognosis.

关 键 词：食管癌 二肽基肽酶Ⅲ 第二个线粒体衍生的半胱氨酸蛋白酶激活剂 临床病理特征 预后 

分 类 号：R735.1[医药卫生—肿瘤]