EP300调控肝癌细胞侵袭迁移的机制  

Mechanism of EP300 regulation of invasion and metastasis of hepatocellular carcinoma cells

作　　者：陈攀[1] 郭文超 谢威[2] 石磊[2] 权虎[2] 罗嘉[2] 陈杰[2,3] CHEN Pan;GUO Wenchao;XIE Wei;SHI Lei;QUAN Hu;LUO Jia;CHEN Jie(Animal Laboratory Center,Hunan Cancer Hospital,Changsha,410013,Hunan,China;Department of Hepatobiliary Enterosurgery,Hunan Cancer Hospital,Changsha,410013,Hunan,China;Tulufan People’s Hospital,Tulufan,838000,Xinjiang,China)

机构地区：[1]湖南省肿瘤医院动物实验中心,湖南长沙410013 [2]湖南省肿瘤医院肝胆肠外科,湖南长沙410013 [3]吐鲁番市人民医院,新疆吐鲁番838000

出　　处：《肿瘤药学》2023年第6期714-718,共5页Anti-Tumor Pharmacy

基　　金：湖南省自然科学基金项目(2022JJ70020);湖南省临床医疗技术创新引导项目(2021SK51115);湖南省卫健委课题(20201163,20200545);湖南省肿瘤医院科研攀登计划(ZX2021003)。

摘　　要：目的 探讨组蛋白乙酰基转移酶家族成员腺病毒E1A结合蛋白P300(EP300)调控肝癌细胞侵袭、迁移的作用及机制。方法 收集我院45例肝癌患者的癌旁组织与癌组织,利用qRT-PCR检测EP300在组织中的表达。在肝细胞癌细胞系HepG2中构建EP300稳定干扰细胞系,细胞划痕实验和Transwell实验观察干扰EP300表达对HepG2细胞侵袭和转移的影响。Western blotting分析EP300对WNT通路的影响。结果 与癌旁组织相比,EP300在肝癌组织中的表达明显增加。与对照组相比,EP300低表达HepG2细胞划痕愈合更慢(P<0.05),穿膜细胞明显减少(P<0.05),SNAIL表达明显减少,E-cadherin表达明显增加。结论 EP300能够通过激活WNT通路促进肝癌细胞侵袭和迁移。Objective To investigate the effect and mechanism of adenovirus E1A binding protein P300(EP300),a member of histone acetyltransferase family,on the invasion and metastasis of hepatocelluar carcinoma cells.Methods The paracancer tissues and tumor tissues of 45 patients with hepatocelluar carcinoma in our hospital were collected,and the expression of EP300 in tissues were further verified by qRT-PCR.EP300 stable interfering cell lines were constructed in hepatocellular carcinoma cell line HepG2.The effects of interfering EP300 on the invasion and metasta-sis of HepG2 cells were observed by cell scratch assay and Transwell assay.The effects of EP300 on WNT pathway was analyzed by Western blotting.Results The expression of EP300 in hepatocellular carcinoma was significantly in-creased as compared with that in adjacent tissues(P<0.05).Moreover,lowly-espressed EP300 could inhibit the cells scratch healing and invasion.After downregulation of EP300 expression,the SNAIL expression decreased significant-ly,while the E-cadherin expression increased significantly.Conclusion EP300 can promote the invasion and metasta-sis of hepatocelluar carcinoma cells by activating WNT pathway.

关 键 词：EP300 肝癌 侵袭 迁移 

分 类 号：R735.7[医药卫生—肿瘤]