口腔上皮细胞具核梭杆菌表达与口腔鳞状细胞癌患者病情及预后的关系  

Relationship between Fusobacterium nucleatum expression in oral epithelial cells and the condition and prognosis of patients with oral squamous cell carcinoma

作　　者：周丽凤 蒋霞[2] 张蓉蓉[1] 王丽[1] 梁巧梅 ZHOU Li-feng;JIANG Xia;ZHANG Rong-rong;WANG Li;LIANG Qiao-mei(Department of Stomatology,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,Hunan,China;不详)

机构地区：[1]湖南中医药大学第二附属医院口腔科,湖南长沙410005 [2]湖南中医药大学第二附属医院重症医学科,湖南长沙410005

出　　处：《广东医学》2023年第12期1554-1559,共6页Guangdong Medical Journal

基　　金：湖南省卫生健康委科研项目(202232102017)。

摘　　要：目的探讨口腔上皮细胞具核梭杆菌预测口腔鳞状细胞癌(OSCC)患者的预后价值。方法收集湖南中医药大学第二附属医院2018年12月至2022年4月151例OSCC的患者,实时定量荧光定量PCR对具核梭杆菌(Fn)基因序列进行定量。LPS和CD163免疫染色剂检测革兰氏阴性菌和巨噬细胞。评估肿瘤内Fn与两个独立的OSCC患者队列中的临床病理特征,复发和总生存期(OS)之间的关系,并探索与免疫相关基因的相互作用。结果Fn阳性与年龄较大、饮酒和饮酒加吸烟摄入较少以及淋巴结浸润频率较低有关;与Fn阴性肿瘤相比,转移性复发较少,OS、无复发和无转移生存期明显延长,免疫相关基因和免疫组织化学分析表明,Fn阳性与M2巨噬细胞呈负相关,差异有统计学意义(P<0.05)。结论Fn相关性OSCC具有特定的免疫微环境,在老年非饮酒患者中更常见,并且预后良好。Objective To investigate the prognostic value of Fusobacterium nucleatum in oral epithelial cells in patients with oral squamous cell carcinoma(OSCC).Methods A total of 151 patients with OSCC treated at the Second Affiliated Hospital of Hunan University of Chinese Medicine from December 2018 to April 2022 were included.Real-time quantitative fluorescence PCR was used to quantify the Fusobacterium nucleatum(Fn)gene sequences.Gram-negative bacteria and macrophages were detected using LPS and CD163 immunostaining.The correlation between tumor-associated Fn and clinical pathological characteristics,recurrence,and overall survival(OS)was assessed in two independent OSCC patient cohorts.Interactions with immune-related genes were explored.Results Fn positivity was associated with older age,less alcohol consumption,and lower rates of alcohol and smoking intake,as well as lower frequency of lymph node infiltration.Compared to Fn-negative tumors,Fn-positive tumors had fewer metastatic recurrences,significantly prolonged OS,recurrence-free survival,and metastasis-free survival.Immunohistochemical analysis and immune-related gene analysis revealed a negative correlation between Fn positivity and M2 macrophages,with statistically significant differences(P<0.05).ConclusionFn-associated OSCC exhibits a specific immune microenvironment,is more common in elderly non-drinking patients,and has a favorable prognosis.

关 键 词：口腔上皮细胞 具核梭杆菌 口腔鳞状细胞癌 

分 类 号：R780.1[医药卫生—口腔医学] R739.85[医药卫生—临床医学]