艾司奥美拉唑对大鼠肠道菌群结构组成的影响  

Effect of esomeprazole on intestinal microbiota structure and composition in rats

作　　者：贾茹[1] 魏世杰[1] 陈文华[1] 贺少龙 付岩[1] JIA Ru;WEI Shi-jie;CHEN Wen-hua;HE Shao-long;FU Yan(Department of Pharmacy,General Hospital of Ningxia Medical University,Yinchuan 750004,Ningxia China)

机构地区：[1]宁夏医科大学总医院药剂科,宁夏银川750004

出　　处：《肠外与肠内营养》2023年第6期375-380,共6页Parenteral & Enteral Nutrition

基　　金：宁夏自然科学基金(2019AAC03244)。

摘　　要：目的:研究艾司奥美拉唑(EMZ)对肠道菌群结构和组成的影响。方法:将7只大鼠置于代谢笼中饲养,灌胃EMZ(3.6 mg/kg体质量),每日1次,连续灌胃14 d。分别在EMZ给药前及给药后两周收集大鼠新鲜粪便,应用16SrRNA高通量测序,确定粪便细菌组成。结果:EMZ灌胃两周后大鼠肠道菌群组成发生明显改变,肠单胞菌属(Intestinimonas)、嗅杆菌属(Odoribacter)和卵形拟杆菌(Bacteroides-ovatus)相对丰度较给药前明显升高(P<0.05);与此相反,米氏链球菌(Streptococcus_merionis)、萨特氏科(Sutterellaceae)、副拟杆菌属(Parasutterella)、假黄酮素属(Pseudoflavonifractor)、醋酐菌属(Acetanaerobacterium)、瘤胃球杆菌(Rumenbacterium)和厚壁杆菌属(Firmicutes_bacterium),给药两周后相对丰度较给药前明显降低(P<0.05)。在科水平,给药两周后瘤胃球菌科细菌相对丰度明显低于给药前(P<0.05)。EMZ给药后肠道细菌多样性和丰富度均较给药有所降低,但无显著性差异(P>0.05)。结论:EMZ处理可导致肠道菌群组成变化,对肠道菌群多样性无明显影响。使用EMZ两周会引起一些肠道共生菌相对丰度改变,如瘤胃球菌等相对丰度降低,可能导致肠道细菌感染的风险增加。Objective:This study aims to investigate the effects of esomeprazole(EMZ)on the gut microbiota structure and composition in rats.Methods:Seven rats were administered intragastrically with EMZ(3.6mg·kg-1·d-1)for 14 days.The fecal samples were freshly collected from the rats before and after EMZ administration,respectively.DNA was extracted from the fecal samples,and 16SrRNA amplicons were obtained and sequenced through the highthroughput sequencing plate(NovaSeq 6000).Results:The gut microbiota was significantly altered at 14 days post EMZ administration.The relative abundance in Intestinimonas,Odoribacter,and Bacteroides-ovatus in the fecal microbiota was dramatically enhanced at 14 days compared with those of the pre-treatment(P<0.05).By contrast,the relative abundance of some bacterial taxons,such as Streptococcus_merionis,Sutterellaceae,Parasutterella,Pseudoflavonifractor,Acetanaerobacterium,Rumen_bacterium and Firmicutes_bacterium,was remarkably reduced at 14days(P<0.05,vs.pre-treatment).At 14 days pos tadministration,he relative abundance of the class Ruminococcaceae was significantly reduced compared that of pre-treatment(P<0.05).Tthe richness and diversity of the intestinal microbiota showed slight reductions,while no significant differences were seen between pre-and post treatment(P>0.05).Conclusions:EMZ administration could lead to significant changes in the microbiota composition,but not in the alpha-diversity.Administration of EMZ for 2 weeks could induce alterations in the relative abundance of some certain bacteria,such as Ruminococcus,which may cause increased risk in intestinal infection.

关 键 词：艾司奥美拉唑 肠道菌群 微生物 16SrRNA测序 

分 类 号：R574[医药卫生—消化系统]