Probing mitochondrial damage using a fluorescent probe with mitochondria-to-nucleolus translocation  

作　　者：Chi Li Chong Zong Yang Liu Zhiqiang Liu Kang-Nan Wang Xiaoqiang Yu 

机构地区：[1]State Key Laboratory of Crystal Materials,Shandong University,Ji'nan 250100,China [2]Shenzhen Research Institute of Shandong University,Shenzhen 518057,China

出　　处：《Chinese Chemical Letters》2024年第1期352-356,共5页中国化学快报（英文版）

基　　金：the Shenzhen Science and Technology Research and Development Funds(No.JCYJ20190806155409104);National Natural Science Foundation of China(Nos.52150222,21672130 and 52073163);Guangdong Basic and Applied Basic Research Foundation(No.2019A1515110356);the Qilu Young Scholars Program of Shandong University.

摘　　要：Mitochondrial damage is closely related to the occurrence of many diseases.However,accurate monitoring and reporting of mitochondrial damage are not easy.Here,we developed a small molecule fluorescent probe named CB-Cl,which has splendid spectral properties(large Stokes shift,strong affinity for RNA,etc.)and excellent targeting ability to intracellular mitochondria.After mitochondria were damaged by external stimuli,CB-Cl would light up the nucleolus as a signal reporter.The cascade imaging of mitochondria and nucleolus using CB-Cl can monitor and visualize the mitochondrial status in living cells in real-time.Based on the above advantages,the probe CB-Cl has reference significance for the related research of mitochondrial damage and the prevention and treatment of related diseases.

关 键 词：Fluorescent probe Mitochondria targeting Nucleolus translocation Mitochondrial damage diagnosis Fluorescence imaging 

分 类 号：R36[医药卫生—病理学] O657.3[医药卫生—基础医学]