聚四氟乙烯膜改性个性化钛网的制备及其抗菌性能研究  

Study on the preparation and antibacterial properties of polytetrafluoroethylene film modified customized titanium mesh

作　　者：杨波[1] 韩泽奎 代浩然 张亮[1] 臧旖欣 王心彧[1] 宿玉成 Yang Bo;Han Zekui;Dai Haoran;Zhang Liang;Zang Yixin;Wang Xinyu;Su Yucheng(Key Laboratory of Oral Biomaterials and Clinical Applications of Heilongjiang Province,Stomatology Engineering Experimental Center of Jiamusi University,School of Stomatology,Jiamusi University,Jiamusi 154002,Heilongjiang,China;Beijing Citident Hospital of Stomatology,Beijing Implant Training College(BITC),Beijing 100032,China;Dental Implant Center,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100032,China)

机构地区：[1]黑龙江省生物医学材料及临床应用重点实验室、佳木斯大学口腔医学工程实验中心、佳木斯大学口腔医学院,154002 [2]北京瑞城口腔医院北京口腔种植培训中心(BITC),100032 [3]中国医学科学院北京协和医院口腔种植中心,100032

出　　处：《中国口腔种植学杂志》2023年第6期451-459,共9页Chinese Journal of Oral Implantology

摘　　要：目的通过在个性化钛网表面制备聚四氟乙烯膜,改善个性化钛网表面生物相容性和抗菌性能。方法采用白刚玉对个性化钛网表面进行喷砂粗化并空气喷涂聚四氟乙烯分散液,烧结热固化成膜以获得聚四氟乙烯膜改性个性化钛网(PMTM)。实验分组为激光选区熔融打印未处理(CON)、机械抛光(MP)、低粒径喷砂(SB)、成品钛加强致密性聚四氟乙烯膜(TRDP),分析试样表面形貌、粗糙度、亲水性、官能团、物相组成。对与金黄色葡萄球菌和大肠杆菌共培养后各组试样表面黏附的细菌数量及形态进行分析,评估聚四氟乙烯膜改性个性化钛网的体外抗菌能力。结果PMTM组表面形成稳定结合、高纯度的聚四氟乙烯薄膜,使改性的个性化钛网表面微米尺度粗糙度下降,高度疏水。CON组表面细菌量最多(P<0.001),MP、SB组表面细菌黏附数量较低。PMTM及TRDP组细菌黏附量相对最低,两者抗细菌黏附效果相近(P>0.05)。结论在个性化钛网表面制备聚四氟乙烯膜可以抑制细菌黏附,是一种较为理想的个性化钛网表面处理手段。Objective By preparing polytetrafluoroethylene film on the surface of customized titanium mesh,the biocompatibility and antibacterial ability of the surface of customized titanium mesh were improved.Methods The surface of the customized titanium mesh was sandblasted to roughen the surface by alumina and air-sprayed with PTFE dispersion.The polytetrafluoroethylene film modified customized titanium mesh(PMTM)was obtained by sintering and heat curing.The experimental groups were selective laser melting printing untreated group(CON),mechanical polishing group(MP),lowgrit sandblasting group(SB),titanium-reinforced dense polytetrafluoroethylene film group(TRDP).The surface morphology,roughness,hydrophilicity,functional groups and phase composition of the samples were analyzed.The number and morphology of bacteria adhering to the surface of each group of samples after co-culture with Staphylococcus aureus and Escherichia coli were analyzed to evaluate the in vitro antibacterial ability of polytetrafluoroethylene film modified customized titanium mesh.Results The polytetrafluoroethylene film with stable bonding and a high purity is formed on the surface of the PMTM group,which reduced the micron-scale roughness and is highly hydrophobic.The number of bacteria on the surface of the CON group was the largest(P<0.001),and the numbers of bacteria on the surface of the MP and SB groups were lower.The PMTM and TRDP groups had the lowest bacterial adhesion,and their antibacterial adhesion effects were similar(P>0.05).Conclusion The preparation of polytetrafluoroethylene film on the surface of customized titanium mesh can improve its ability to inhibit bacterial adhesion,which is an ideal surface treatment method for customized titanium mesh.

关 键 词：个性化钛网 聚四氟乙烯 引导骨再生 表面处理 抗菌性能 

分 类 号：TB383.2[一般工业技术—材料科学与工程] R783.1[医药卫生—口腔医学]