Argonaute 2和微小RNA-145-5p对肝癌细胞增殖和转移的影响  

Effects of Ago2 and microRNA-145-5p on the proliferation and metastasis of liver cancer cells

作　　者：李威威 赵杰[1] Li Weiwei;Zhao Jie(Department of Gastroenterology and Hepatobiliary Surgery,Shangqiu First People’s Hospital/Shangqiu Clinical College,Xuzhou Medical University,Shangqiu 476100,China)

机构地区：[1]商丘市第一人民医院/徐州医科大学商丘临床学院胃肠肝胆外科,商丘476000

出　　处：《中华实验外科杂志》2023年第12期2491-2494,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨Ago2和微小RNA(miRNA,miR)-145-5p对肝癌细胞增殖和转移的影响。方法选取2018年6月至2022年12月商丘市第一人民医院收治的98例肝癌患者肿瘤组织和癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)和蛋白质免疫印迹分析miR-145-5p和Ago2表达水平。采用慢病毒在人肝癌细胞系HepG2建立miR-145-5p过表达细胞系,分别为miRNA对照组和miR-145-5p组,采用蛋白质印迹法(Western blot)分析Ago2表达水平;采用慢病毒构建Ago2敲降细胞系,为对照组和Ago2组,采用荧光定量PCR分析miR-145-5p表达水平。采用细胞计数试剂盒(CCK-8)分析不同细胞的增殖能力;采用划痕实验和Transwell实验分析不同细胞的迁移和侵袭能力;分析Ago2和miR-145-5p表达的关系。组间比较采用t检验。结果肝癌组织中Ago2蛋白表达水平(1.99±0.18)高于癌旁组织(1.20±0.12),差异有统计学意义(t=25.260,P<0.05)。肝癌组织中miR-145-5p表达水平(0.64±0.13)低于癌旁组织(1.04±0.15),差异有统计学意义(t=14.060,P<0.05)。miRNA对照组细胞48 h吸光度(A)值(2.01±0.12)高于miR-145-5p组(1.45±0.13),差异有统计学意义(t=7.616,P<0.05)。对照组细胞48 h A值(1.90±0.15)低于Ago2组(2.44±0.13),差异有统计学意义(t=6.603,P<0.05)。miRNA对照组划痕愈合率[(72.74±3.06)%]高于miR-145-5p组[(51.50±5.85)%],差异有统计学意义(t=7.879,P<0.05)。对照组细胞划痕愈合率[(76.31±3.95)%]低于Ago2组[(88.69±2.63)%],差异有统计学意义(t=6.387,P<0.05)。miRNA对照组侵袭数量[(97.17±9.02)个]高于miR-145-5p组[(77.50±6.98)个],差异有统计学意义(t=4.224,P<0.05)。对照组细胞侵袭数量[(93.17±8.18)个]低于Ago2组[(132.83±5.34)个],差异有统计学意义(t=9.941,P<0.05)。Ago2是miR-145-5的靶基因。miRNA对照组Ago2蛋白表达水平(1.46±0.14)高于miR-145-5p组(0.74±0.12),差异有统计学意义(t=9.625,P<0.05)。结论miR-145-5p调节Ago2蛋白表达水平,进而调节肝癌细胞的增殖和�Objective To investigate the effects of Ago2 and microRNA(miRNA,miR)-145-5p on the proliferation and metastasis of liver cancer cells.Methods 98 liver cancer patients with tumor and adjacent tissues admitted to our hospital from June 2018 to December 2022 were selected as the research subjects.The expression levels of miR-145-5p and Ago2 were analyzed using fluorescence quantitative polymerase chain reaction(PCR)and protein immunoblotting.Using lentivirus to establish miR-145-5p overexpression cell lines in human liver cancer cell line HepG2,divided into miRNA control group and miR-145-5p group.The Ago2 expression level was analyzed by Western blotting.The control group and Ago2 group were constructed using Ago2 overexpression lentivirus.The miR-145-5p expression levels were analyzed by fluorescence quantitative PCR.The proliferation ability of different cells were analyzed by cell counting kit-8(CCK-8).The migration and invasion abilities of different cells were analyzed by wounding healing and Transwell experiments.The relationship between miR-145-5p and Ago2 expression was analyzed.The comparison of measurement data between groups was conducted using t-test.Results The expression level of Ago2 protein in liver cancer tissue(1.99±0.18)was significantly higher than that in adjacent tissues(1.20±0.12,t=25.260,P<0.05).The expression level of miR-145-5p in liver cancer tissue(0.64±0.13)was significantly lower than that in adjacent tissues(1.04±0.15,t=14.060,P<0.05).The 48 hour absorbance(A)value of cells in the miRNA control group(2.01±0.12)was significantly higher than that in the miR-145-5p group(1.45±0.13,t=7.616,P<0.05).The 48 hour A value of the control group cells(1.90±0.15)was significantly lower than that of the Ago2 group cells(2.44±0.13,t=6.603,P<0.05).The wound healing rate in the miRNA control group[(72.74±3.06)%]was significantly higher than that in the miR-145-5p group[(51.50±5.85)%,t=7.879,P<0.05].The wound healing rate of the control group cells[(76.31±3.95)%]was significantly lower than

关 键 词：微小RNA-145-5p Ago2 肝癌 增殖 转移 

分 类 号：R735.7[医药卫生—肿瘤]