基于超高效液相色谱三重四极杆质谱法同时检测佛手中香豆素类化合物含量  

A rapid ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry method for simultaneous detection of coumarins in Citrus medica L.var.sarcodactylis swingle

作　　者：周胡怿 赵希娟 焦必宁[1,2,3] ZHOU Huyi;ZHAO Xijuan;JIAO Bining(Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China;Laboratory of Quality and Safety Risk Assessment for Citrus Products,Ministry of Agriculture and Rural Affairs,Chongqing 400712,China;Quality Supervision and Testing Center for Citrus and Seedling,Ministry of Agriculture and Rural Affair,Chongqing 400712,China;College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China)

机构地区：[1]中国农业科学院/西南大学柑桔研究所,重庆400712 [2]农业农村部柑桔产品质量安全风险评估实验室,重庆400712 [3]农业农村部柑桔及苗木质量监督检验测试中心,重庆400712 [4]西南大学园艺园林学院,重庆400715

出　　处：《食品与发酵工业》2024年第2期288-295,共8页Food and Fermentation Industries

基　　金：国家现代农业(柑桔)产业技术体系建设专项(CARS-26);国家农产品质量安全风险评估项目(GJFP20210204)。

摘　　要：佛手作为一种药食两用的柑桔类水果,香豆素类化合物是其关键的生物活性成分之一。由于香豆素类化合物结构相似,并且有同分异构体,目前还没有快速准确的方法针对香豆素类化合物进行同时定量。该研究建立了一种超高效液相色谱三重四极杆质谱法(ultra-performance liquid chromatography tandem triple quaternary mass spectrometry,UPLC-QqQ-MS/MS),在14 min内定性并定量4个不同产地佛手柑中的41种香豆素类化合物,包括16种简单香豆素、23种呋喃香豆素和2种其他香豆素,其中有8组同分异构体。该方法灵敏度高、准确度高、精确度高,可用于佛手实际样品中香豆素的筛选和测定,这是迄今为止筛查并定量香豆素最全面和快速的方法。柠檬内酯是佛手中含量最高的香豆素,浙江佛手中检出的香豆素类化合物含量和种类均最多,并且有4种香豆素在佛手中首次检出。Citrus medica L.var.sarcodactylis swingle can be used as both food and medicine,and coumarins are one of its key bioactive components.There is currently no accurate method for the quick detection and comprehensive quantitation of a series of coumarins owing to their structural similarity and some isomers.In this study,an ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry(UPLC-QqQ-MS/MS)approach was developed for the qualitative and quantitative detection of 41 coumarins in Citrus medica L.var.sarcodactylis swingle.In brief,within a 14-minute chromatographic run,16 simple coumarins,23 furocoumarins,and 2 other coumarins,especially 8 groups of isomers,were separated simultaneously.Recoveries,linear correlation coefficients,detection and quantitation limits,as well as inter-day and intra-day precisions,were used to validate this newly developed method.The method can be utilized for the screening and measurement of coumarins in Citrus medica L.var.sarcodactylis swingle because it has been verified to have high sensitivity,accuracy,and good recoveries.Four coumarins including 8-geranyloxypsoralen,bergamottin,isopimpinellin,and meranzin hydrate were reported for the first time in the fingered citron fruits.This is by far the most comprehensive and quick method to identify and determine coumarins in fingered citrons.

关 键 词：佛手 香豆素 超高效液相色谱串联三重四极杆质谱法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]