miR-552-3p通过PTEN/AKT途径促进子宫内膜癌细胞体外增殖和转移  

miR-552-3p promotes proliferation and metastasis of endometrial cancer cells in vitro through PTEN/AKT pathway

作　　者：姜方清 秦丽 JIANG Fangqing;QIN Li(Department of Gynecology and Obstetrics,The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院妇产科,湖北恩施445000

出　　处：《生物技术》2023年第6期738-744,共7页Biotechnology

摘　　要：[目的]探讨miR-552-3p与PTEN/AKT信号通路对子宫内膜癌细胞生物活性的作用机制。[方法]将RL95-2细胞分为两组:inhibitor NC组(NC组)和miR-552-3p inhibitor组(inhibitor组);或Si NC组和Si PTEN组。通过MTT检测细胞活性,流式细胞术检测细胞凋亡率,Transwell小室检测细胞侵袭数目,划痕实验检测细胞迁移距离,免疫印迹检测细胞相关蛋白水平。[结果]与hEEC细胞比较,miR-552-3p在肿瘤细胞表达异常升高(0.62±0.03 vs 1.56±0.07);和转染inhibitor NC相比,转染miR-552-3p inhibitor后,RL95-2细胞的活性降低,RL95-2细胞的迁移和侵袭能力显著降低(25.29%±2.89%vs 11.27%±1.23%;65.33±3.12 vs 40.27±8.03),PTEN蛋白表达降低(0.93±0.12 vs 0.67±0.03),CCS-3和Bax的表达增加(0.97±0.11 vs 1.72±0.09,1.01±0.10 vs3.03±0.09)。和转染Si NC相比,转染Si PTEN后,RL95-2细胞的活性被明显抑制,细胞凋亡明显增加(11.02%±0.35%vs 30.89%±1.23%),CCS-3和Bax的表达增加(0.23±0.21 vs 1.09±0.08,0.21±0.31 vs 0.82±0.05)。双荧光素酶报告基因实验表明,相较于inhibitor NC组细胞,miR-552-3p innhibitor组细胞PTEN-WT报告基因的荧光素酶活性显著降低(0.93±0.09 vs 0.59±0.05)。[结论]抑制miR-552-3p能够抑制子宫内膜癌细胞的生物活性,这一过程与miR-552-3p调控PTEN/AKT通路有关。[Objective]To investigate the mechanism of miR-552-3p and PTEN/AKT signaling pathway on the biological activity of endometrial cancer cells.[Method]RL95-2 cells were divided into two groups:inhibitor NC group(NC group)and miR-552-3p inhibitor group(inhibitor group);or Si NC group and Si PTEN group.Cell viability was detected by MTT,cell apoptosis rate was detected by flow cytometry,cell invasion number was detected by Transwell chamber,cell migration distance was detected by scratch assay,and cell-related protein levels were detected by Western Blotting.[Result]Compared with hEEC cells,miR-552-3p expression was abnormally elevated in tumor cells(0.62±0.03 vs 1.56±0.07);compared with transfection of inhibitor NC,the activity of RL95-2 cells after transfection with miR-552-3p inhibitor decreased,and the migration and invasion ability of RL95-2 cells were significantly reduced(25.29%±2.89%vs 11.27%±1.23%;65.33±3.12 vs 40.27±8.03),the PTEN protein expression was decreased(0.93±0.12 vs 0.67±0.03),CCS-3 and Bax expression was increased(0.97±0.11 vs 1.72±0.09,1.01±0.10 vs 3.03±0.09).Compared with transfection with Si NC,transfection with Si PTEN significantly inhibited the activity of RL95-2 cells,significantly increased apoptosis(11.02%±0.35%vs 30.89%±1.23%),increased expression of CCS-3 and Bax(0.23±0.21 vs 1.09±0.08,0.21±0.31 vs 0.82±0.05).Dual luciferase reporter gene assay showed that luciferase activity of PTEN-WT reporter gene was significantly reduced in miR-552-3p innhibitor group cells compared to innhibitor NC group cells(0.93±0.09 vs 0.59±0.05).[Conclusion]Inhibition of miR-552-3p can inhibit the biological activity of endometrial cancer cells,and this process is related to the regulation of PTEN/AKT pathway by miR-552-3p.

关 键 词：miR-552-3p PTEN/AKT RL95-2 增殖 转移 子宫内膜癌 

分 类 号：R737.33[医药卫生—肿瘤]