花生四烯酸调控网络在贻贝麻痹性贝毒代谢中的作用——基于转录组和代谢组联合分析  

The role of arachidonic acid regulatory network in the metabolism of paralytic shellfish toxins in Mytilus galloprovincialis:based on combined transcriptome and metabolome analysis

作　　者：张倩茹 谭志军[2,3] 郑关超 董晨帆 杨越聪 吴海燕 Zhang Qianru;Tan Zhijun;Zheng Guanchao;Dong Chenfan;Yang Yuecong;Wu Haiyan(School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisher-ies Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China)

机构地区：[1]江苏海洋大学食品科学与工程学院,江苏连云港222005 [2]中国水产科学研究院黄海水产研究所农业部水产品质量安全检测与评价重点实验室,山东青岛266071 [3]中国水产科学研究院黄海水产研究所海水养殖生物育种与可持续产出全国重点实验室,山东青岛266071

出　　处：《海洋学报》2023年第11期142-152,共11页

基　　金：国家重点研发计划项目(2022YFC3105203);国家自然科学基金面上项目(32072329);国家现代农业产业技术项目(CARS49);中国水产科学研究院基本科研业务费项目(2023TD76)。

摘　　要：紫贻贝(Mytilus galloprovincialis)是富集麻痹性贝类毒素(Paralytic Shellfish Toxins,PSTs)能力很强的代表性双壳贝类,但研究表明紫贻贝暴露于PSTs也会引起机体炎症反应,其作用机理及对毒素代谢影响尚不清楚。本研究采用转录组学与代谢组学联合分析技术,比较了产PSTs的链状亚历山大藻(Alexandriumcatenella)暴发的不同时期,紫贻贝机体的基因表达量与代谢物含量差异,以解析PSTs胁迫下紫贻贝机体的炎症反应机制。结果表明,紫贻贝暴露于PSTs后表达的基因和代谢物均发生显著变化,其中差异表达基因17232个,差异代谢物341个。基于联合分析,差异表达基因与差异代谢物显著富集在花生四烯酸和谷胱甘肽代谢通路中,基因PLA2G2F、ALOX5、TBXAS1以及代谢物ARA、PGH2、TXA2、LTA4、5-HETE对贻贝机体的促炎反应发挥重要作用;而基因GPX4、CYP2J2和代谢物15-HETE、GSH则调节机体炎症的消退。本研究揭示花生四烯酸相关通路在贻贝机体炎症反应过程中具有重要的调控作用,为下一步深度揭示PSTs胁迫下贻贝机体炎症网络化响应机制提供了研究基础。Mytilus galloprovincialis is the representative bivalve with the great ability to enrich for paralytic shell-fish toxins(PSTs),however,it has been shown that exposure of Mytilus galloprovincialis to PSTs can induces an inflammatory response in the organism,while its mechanism and effects on toxin metabolism are not clear.In this study,transcriptomics and metabolomics were used to compare the differences in gene expression and metabolite content of purple mussel(M.galloprovincialis)in different periods of Alexandrium catenella bloom,in order to analyze the inflammatory response mechanism of purple mussel under the stress of PSTs.The results show that there are significantly changes in genes and metabolites expressed after exposure to PSTs,including 17232 differ-entially expressed genes and 341 differentially expressed metabolites.Based on the association analysis,differen-tially expressed genes and metabolites are significantly enriched in the arachidonic acid and glutathione metabolic pathways,and genes PLA2G2F,ALOX5,TBXAS1 and metabolites ARA,PGH2,TXA2,LTA4,5-HETE play import-ant roles in the pro-inflammatory response of the mussel organism;while genes GPX4,CYP2J2 and metabolites 15-HETE and GSH regulate the regression of inflammation in the organism,constituting a complex network of inflam-matory regulatory signals.This study reveals that arachidonic acid-related pathways play an important regulatory role in the inflammatory response of the mussel organism,which provides a basis for further insight into the mech-anism of the inflammatory network response of the mussel organism under the stress of PSTs.

关 键 词：麻痹性贝类毒素 紫贻贝 代谢 花生四烯酸 炎症 

分 类 号：S944.42[农业科学—水产养殖] P714.5[农业科学—水产科学]