miR-223对脂多糖诱导的奶牛乳腺上皮细胞炎症反应的影响  

Effects of miR-223 on lipopolysaccharide-induced inflammatory response of dairy mammary epithelial cells

作　　者：赵天夺 王若薇 张莉[1] 魏祥飞 杨慧琳 符海鑫 王春梅[1] ZHAO Tianduo;WANG Ruowei;ZHANG Li;WEI Xiangfei;YANG Huilin;FU Haixin;WANG Chunmei(The Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2024年第1期28-32,共5页China Dairy Industry

基　　金：国家自然科学基金(31072103)。

摘　　要：以中国荷斯坦奶牛乳腺上皮细胞(dairy cow mammary epithelial cell,DCMECs)为研究模型,探讨mi R-223对脂多糖(lipopolysaccharide,LPS)诱导的DCMECs炎症反应与乳脂合成的影响。应用Western blot检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)与脂肪酸合成酶(fatty acid synthase,FASN)的表达情况,通过脂质体转染技术将mi R-223转染至DCMECs,qRT-PCR检测mi R-223的相对表达量,采用原位荧光技术分别检测DCMECs的凋亡与乳脂合成能力。结果表明LPS诱导DCMECs炎症反应的最佳浓度为1μg/m L;添加LPS可促进DCMECs mi R-223的表达;DCMECs转染mi R-223后,mi R-223的表达显著上调;mi R-223可下调LPS诱导的DCMECs炎症蛋白因子TNF-α,上调乳脂合成相关蛋白FASN,抑制LPS诱导的细胞凋亡,促进LPS诱导的乳脂合成。In this study,Chinese Holstein mammary epithelial cells(DCMECs)were used as a model to investigate the effects of miR-223 on DCMECs inflammation and milk fat synthesis induced by lipopolysaccharide(LPS).Western blot was used to detect the expression of Tumor necrosis factor-α(TNF-α)and Fatty acid synthase(FASN).miR-223 was transfected into DCMECs,qRT-PCR by liposome transfection technique to detect the relative expression of miR-223.In situ fluorescence technique was used to detect the apoptosis and milk fat synthesis of DCMECs.The results showed that the best concentration of LPS to induce DCMECs inflammatory reaction was 1μg/mL;adding LPS could promote the expression of DCMECs miR-223;after DCMECs transfection into miR-223,the expression of miR-223 was significantly up-regulated;miR-223 could down-regulate the inflammatory protein TNF-αof DCMECs induced by LPS,up-regulate the protein FASN related to milk fat synthesis,inhibit LPS-induced apoptosis and promote LPS-induced milk fat synthesis.

关 键 词：奶牛乳腺上皮细胞 miR-223 脂多糖 凋亡 乳脂 

分 类 号：S823[农业科学—畜牧学]