HLA-DM基因多态性与脊髓灰质炎疫苗诱导抗体应答的相关性  

Correlation Analysis of HLA-DM Gene Polymorphisms with Polio Vaccine-induced Antibody Response

作　　者：齐汝楠 史磊[1] 刘舒媛[1] 李菁[1] 史荔[1] QI Runan;SHI Lei;LIU Shuyuan;LI Jing;SHI Li(Institute of Medical Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Kunming Yunnan 650118,China)

机构地区：[1]中国医学科学院&北京协和医学院医学生物学研究所,云南昆明650118

出　　处：《昆明医科大学学报》2024年第1期8-14,共7页Journal of Kunming Medical University

基　　金：脊髓灰质炎系列疫苗研发及sIPV/bOPV联合序贯免疫研究基金资助项目(2015ZX09101031)。

摘　　要：目的 探讨HLA-DM基因多态性与脊髓灰质炎疫苗诱导抗体应答的相关性。方法 选取355名2~3月龄广西壮族自治区的健康婴幼儿为研究对象,采用Sanger测序法对DMA的外显子3和DMB的外显子2/3总共10个SNPs进行基因分型,在等位基因、基因型和单倍型水平上分析DMA和DMB基因与脊髓灰质炎疫苗诱导抗体应答的相关性。结果 脊髓灰质炎疫苗诱导的I型抗体应答中,DMA*01:02、DMB*01:01、DMB*01:01/DMB*01:01和DMA*01:02-DMB*01:01在抗体非阳转组中的频率高于阳转组(P <0.05);脊灰II型抗体应答中,DMA*01:02、DMA*01:02/DMA*01:02、DMB*01:01/DMB*01:01和DMA*01:02-DMB*01:01在抗体非阳转组中的频率高于阳转组(P <0.05)。结论 DMA*01:02和DMB*01:01等位基因可能与脊髓灰质炎疫苗诱导的I型和II型抗体应答有关。Objective To investigate the correlation between HLA-DM gene polymorphism and antibody response induced by poliomyelitis vaccine.Methods 355 healthy infants aged 2 to 3 months in Guangxi Zhuang Autonomous Region were selected as the study objects,and 10 SNPs of DMA exon 3 and DMB exon 2/3 were genotyped by Sanger sequencing.The correlation between DMA and DMB genes and poliomyelitis vaccine-induced antibody response was analyzed at allele,genotype and haplotype levels.Results In the type I antibody response induced by polio vaccine,the frequencies of DMA*01:02,DMB*01:01,DMB*01:01/DMB*01:01 and DMA*01:02-DMB*01:01 were higher in the non-seroconversion group than in the seroconversion group(P<0.05).In the polio type II antibody response,the frequencies of DMA*01:02,DMA*01:02/DMA*01:02,DMB*01:01/DMB*01:01 and DMA*01:02-DMB*01:01 were higher in the non-seroconversion group than in the seroconversion group(P<0.05).Conclusion Alleles DMA*01:02 and DMB*01:01 may be associated with type I and type II antibody responses induced by poliomyelitis vaccine.

关 键 词：HLA-DM 基因多态性 脊髓灰质炎疫苗 抗体应答 

分 类 号：R394.4[医药卫生—医学遗传学]