STEAP1在膀胱移形细胞癌中的表达及其潜在诊断价值  

Expression of STEAP1 in bladder transitional cell carcinoma and its potential diagnostic value

作　　者：万玛草 白洁[2] 赵宪龙 常斌 王建平[3] 杨立昌 WAN Macao;BAI Jie;ZHAO Xianlong;CHANG Bin;WANG Jianping;YANG Lichang(Department of General Medicine,the 940th Hospital of Joint Logistics Support Force of the Chinese People′s Liberation Army,Lanzhou,Gansu 730070,China;Department of Clinical Laboratory,the 940th Hospital of Joint Logistics Support Force of the Chinese People′s Liberation Army,Lanzhou,Gansu 730070,China;Department of Urology,the 940th Hospital of Joint Logistics Support Force of the Chinese People′s Liberation Army,Lanzhou,Gansu 730070,China)

机构地区：[1]中国人民解放军联勤保障部队第九四O医院全科医学科,甘肃兰州730070 [2]中国人民解放军联勤保障部队第九四O医院检验科,甘肃兰州730070 [3]中国人民解放军联勤保障部队第九四O医院泌尿外科,甘肃兰州730070

出　　处：《国际检验医学杂志》2024年第3期272-276,共5页International Journal of Laboratory Medicine

基　　金：甘肃省科技计划项目(21JR7RA005)。

摘　　要：目的探索前列腺六段扩膜上皮抗原1(STEAP1)在膀胱移行细胞癌中的表达及其潜在诊断价值。方法选取2021年6月至2022年12月于中国人民解放军联勤保障部队第九四O医院行外科手术治疗的52例膀胱移行细胞癌患者作为观察组,另外选取同期年龄、性别、基础疾病患病率等基础临床资料匹配的膀胱良性肿瘤患者52例作为对照组。通过酶联免疫吸附试验法、实时荧光定量聚合酶链反应测定两组患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平,以及对比不同病理参数患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平。通过Spearman相关性分析、二元Logistic回归分析筛选膀胱移行细胞癌发生及临床分期的危险因素,通过受试者工作特征(ROC)曲线及曲线下面积(AUC)评价各指标对膀胱移行细胞癌的诊断及预测价值。结果观察组膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平均显著高于对照组,差异有统计学意义(P<0.05)。中晚期膀胱移行细胞癌患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平均显著高于早期膀胱移行细胞癌患者,差异有统计学意义(P<0.05)。通过二元Logistic回归分析表明,患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平增高均是膀胱移行细胞癌发生及中晚期膀胱移行细胞癌的独立危险因素(P<0.05)。ROC曲线分析显示,患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA独立预测膀胱移行细胞癌发生的AUC分别为0.841(95%CI:0.760~0.922,P<0.001)、0.936(95%CI:0.893~0.980,P<0.001),均具有较高的预测效能。结论膀胱移行细胞癌患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平与膀胱移行细胞癌发生及中晚期膀胱移行细胞癌呈正相关,提示STEAP1可作为诊断、预测膀胱移行细胞癌发生、发展的潜在标志物。Objective To investigate the expression and potential diagnostic value of six-transmembrane epithelial antigen of prostate 1(STEAP1)in bladder transitional cell carcinoma.Methods 52 patients with transitional cell carcinoma of the bladder who underwent surgical treatment at the 940th Hospital of Joint Logistics Support Force of the Chinese People′s Liberation Army from June 2021 to December 2022 were selected as the observation group.In addition,52 patients with benign tumors of the bladder who matched basic clinical data such as age,gender,and disease incidence were selected as the control group.The relative expression levels of STEAP1 and STEAP1 mRNA in bladder tumor tissues of patients in the two groups were determined by enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR,and the relative expression levels of STEAP1 and STEAP1 mRNA in bladder tumor tissues of patients with different pathological parameters were compared.Spearman correlation analysis and binary Logistic regression analysis were used to screen the risk factors for the occurrence and clinical stage of bladder transitional cell carcinoma.Receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to evaluate the diagnostic and predictive value of each indicator for bladder transitional cell carcinoma.Results The relative expression levels of STEAP1 and STEAP1 mRNA in bladder tumor tissues in observation group were significantly higher than those in control group,with statistical significance(P<0.05).The relative expression levels of STEAP1 and STEAP1 mRNA in bladder tumor tissues of patients with middle and advanced bladder transitional cell carcinoma were significantly higher than those of patients with early bladder transitional cell carcinoma,with statistical significance(P<0.05).Binary Logistic regression analysis showed that the relative expression level of STEAP1 and STEAP1 mRNA in bladder tumor tissues of patients were independent risk factors for the development of bladder transitional ce

关 键 词：膀胱移行细胞癌 前列腺六段扩膜上皮抗原1 膀胱肿瘤组织 

分 类 号：R737.14[医药卫生—肿瘤]