蔷薇科小G蛋白ROP的鉴定、分类及特征分析  

Identification,Classification and Characterization of Small G Protein ROPs in Rosaceae

作　　者：刘礼瑶 熊仁次[1,2] 何璐 马思洁 杨叔青 LIU Liyao;XIONG Renci;HE Lu;MA Sijie;YANG Shuqing(College of Agriculture,Tarim University,Alar 843300,China;Key Laboratory of Integrated Control of Agricultural Pests in Southern Xinjiang,Alar 843300,China;College of Horticulture and Forestry,Tarim University,Alar 843300,China)

机构地区：[1]塔里木大学农学院,新疆阿拉尔843300 [2]南疆农业有害生物综合治理兵团重点实验室,新疆阿拉尔843300 [3]塔里木大学园艺与林学学院,新疆阿拉尔843300

出　　处：《华北农学报》2023年第S01期8-17,共10页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(32160644);塔里木大学校长基金项目(TDZKBS202101)。

摘　　要：为全面系统地挖掘蔷薇科小G蛋白ROP,在分子水平上为蔷薇科抗病育种提供候选基因。物种系统进化关系分析、ROP蛋白全基因组筛查、Rho功能域分析、蛋白聚类分析及理化性质分析分别运用了NCBI、iTOLv6、GDR、SMART、MEGA 11.0和Maximum-likelihood algorithm1及Protparam和Expasy等数据库及生物信息学在线软件。11种蔷薇科植物聚为6个亚支;11种蔷薇科植物中筛查到80个ROP蛋白;80个蔷薇科ROP蛋白和11个拟南芥ROP蛋白聚为6个进化分支;蛋白长度、等电点、分子量、脂肪指数及不稳定指数分别为143~215 aa、5.59~9.63、15.73599~23.87859 ku、85.28~94.95和27.96~57.14。80个蔷薇科ROP蛋白在草莓中数量最多,甜樱桃最少;蔷薇科ROP蛋白分布于第Ⅳ、Ⅴ和Ⅵ分支中,拟南芥ROP蛋白分布于Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ分支中,即分支Ⅵ为蔷薇科特异性分支,分支Ⅰ、Ⅱ和Ⅲ为拟南芥特异性分支。In order to comprehensively and systematically explore the small G protein ROP of Rosaceae,it provides candidate genes for disease resistance breeding of Rosaceae at the molecular level.Phylogenetic relationship analysis,ROP protein genome-wide screening,Rho functional domain analysis,protein cluster analysis and physicochemical property analysis used NCBI,iTOLv6,GDR,SMART,MEGA 11.0 and Maximum-likelihood algorithm1,Protparam and Expasy databases and bioinformatics online software,respectively.Eleven species of Rosaceae were clustered into 6 subclades;80 ROP proteins were screened in 11 species of Rosaceae;80 Rosaceae ROP proteins and 11 Arabidopsis ROP proteins were aggregated into 6 evolutionary branches;the protein length,isoelectric point,molecular weight,fat index and instability index were 143-215 aa,5.59-9.63,15.73599-23.87859 ku,85.28-94.95 and 27.96-57.14,respectively.80 Rosaceae ROP proteins were the most abundant in Fragaria ananassa and the least in Prunus avium;Rosaceae ROP proteins were distributed in branchesⅣ,ⅤandⅥ,Arabidopsis ROP proteins were distributed in branchesⅠ,Ⅱ,Ⅲ,ⅣandⅤ,that is,branchⅥwas a Rosaceae-specific branch,and branchesⅠ,ⅡandⅢwere Arabidopsis-specific branches.

关 键 词：蔷薇科 小G蛋白ROP 全基因组筛查 Rho功能域 系统进化关系 蛋白理化特征 

分 类 号：S685.12[农业科学—观赏园艺]