Inhibition of proliferation,migration,and invasiveness of bladder cancer cells through SAPCD2 knockdown  

作　　者：CHONG SHEN JIAJUN YAN YU REN ZHIRONG ZHU XIAOLONG ZHANG SHUIXIANG TAO 

机构地区：[1]Department of Urology,Shaoxing People’s Hospital,Shaoxing Hospital,Zhejiang University School of Medicine,Shaoxing,China

出　　处：《BIOCELL》2024年第1期97-109,共13页生物细胞（英文）

基　　金：supported by the Medical and Health Science and Technology Program of Zhejiang Province(No.2021KY367).

摘　　要：Introduction:Bladder cancer(BC)has a high incidence and mortality rate worldwide.Suppressor anaphasepromoting complex domain containing 2(SAPCDC2)is over-expressed in a variety of tumors.Objectives:This study investigated the effects of SAPCD2 knockdown on BC cells.Methods:T24 and UMUC3 cell models and the xenografted BC tumor model with SAPCD2 knockdown were established to observe the malignant phenotype of BC cells by cell counting kit-8 assay,colony formation test,wound healing,and Transwell assay,mRNA and proteins expressions were measured with quantitative real-time polymerase chain reaction,western blotting,and tissue immunohistochemistry.Lithium chloride agonist on the Wnt/β-catenin pathway was used to clarify the molecular mechanism of SAPCD2 knockdown.Results:SAPCD2 expression was significantly higher in BC cell lines than in SVHUC-1 cells.SAPCD2 knockdown inhibited viability and cloning,hindered the G0/G1 phase of the cell cycle in UMUC3 and T24 cells,and decreased the migration and invasiveness of BC cells.SAPCD2 knockdown inhibited expression levels of cyclin D1,cyclin B1,N-cadherin,vimentin,Snail,β-catenin,c-Myc,and cyclin-dependent kinase 4,while the P21 and E-cadherin were raised by SAPCD2 knockdown.Furthermore,lithium chloride reversed the effects of SAPCD2 knockdown on the expression levels of the above proteins in UMUC3 and T24 cells.In vivo,SAPCD2 knockdown inhibited the volume,weight,and expression of Ki-67 andβ-catenin in tumors and increased the E-cadherin expression.Conclusion:SAPCD2 knockdown inhibits the malignant phenotype of BC via a pathway involvingβ-catenin.

关 键 词：Bladder cancer SAPCD2 Β-CATENIN C-MYC CDK4 Lithium chloride 

分 类 号：R737.14[医药卫生—肿瘤]