精氨酸对变异链球菌生物膜及牙骨质再矿化作用的体外研究  

Effects of arginine on Streptococcus mutans biofilm and remineralization of artificial cementum lesions:in vitro study

作　　者：王素苹 方立新 陶地豪 张宜爽 郭安迪 刘飞[1] WANG Suping;FANG Lixin;TAO Dihao;ZHANG Yishuang;GUO Andi;LIU Fei(Department of Stomatology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;College of Stomatology,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院口腔内科,郑州450052 [2]郑州大学口腔医学院,郑州450052

出　　处：《郑州大学学报（医学版）》2024年第1期67-71,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81900993);河南省医学科技攻关计划省部共建重点项目(SBGJ202102162);河南省教育厅高等学校重点科研项目(22A320006);河南省重点研发与推广专项(科技攻关)(212102310595,222102310407)。

摘　　要：目的:观察精氨酸对变异链球菌生物膜和牙骨质再矿化的作用,探讨精氨酸对根面龋预防的临床效果及可行性。方法:以不加精氨酸为对照组,采用4种不同质量浓度的精氨酸溶液处理变异链球菌生物膜,研究精氨酸在龋病预防中的效果。24 h后通过pH值测定、结晶紫染色,观察不同质量浓度精氨酸溶液对变异链球菌生物膜的影响。收集无龋坏的人前磨牙制备牙骨质块,构建人工根面龋模型并随机分为对照组(人工唾液处理)、实验组(7.5、15.0和30.0 g/L精氨酸处理)共4组,经梯度精氨酸溶液处理后,进行20 d再矿化实验,通过扫描电镜、能谱仪观察精氨酸对脱矿牙骨质物理及化学构成的影响,分析精氨酸对根面龋早期的治疗效果。结果:相较于对照组,精氨酸处理对变异链球菌生物膜具有明显的抑制效果(P<0.001);根面牙骨质表面结构更为致密,Ca、P含量升高(P<0.001);与低质量浓度精氨酸相比,高质量浓度精氨酸组对生物膜的抑制和根面龋的再矿化作用更为显著。结论:精氨酸对变异链球菌生物膜形成有良好的抑制作用,且能促进根面龋牙骨质再矿化,在根面龋的预防及早期治疗方面效果显著。Aim:To observe the effects of arginine on Streptococcus mutans(S.mutans)biofilm and remineralization of cementum,and explore the clinical effects and feasibility of arginine on the prevention of root caries.Methods:Different concentrations of arginine were added to the culture medium to detect the effect of arginine on S.mutans biofilm growth.The culture medium without arginine served as control group.After 24 hours,pH determination and crystal violet staining were used to detect the effect of arginine on S.mutans biofilm growth.Cementum blocks were prepared from isolated human permanent premolars and made cementum root caries model.They were divided into 4 groups:control group(artificial saliva immersion)and 3 experimental groups(7.5,15.0,and 30.0 g/L arginine solution immersion).After treatment with gradient arginine solutions and 20-day remineralization experiment,physical and chemical compositions of cementum blocks were detected by scanning electronic microscope and energy dispersive spectrometer.Results:Compared with control group,arginine treatment showed obvious inhibitory effect on S.mutans biofilm(P<0.001),the surface structure of the root cementum was denser and the contents of Ca and P increased(P<0.001),and compared with low concentration arginine,high concentration arginine had more significant effects on inhibition of biofilm and remineralization of root caries.Conclusion:Arginine could inhibit S.mutans biofilm formation and promote root caries cementum remineralization,which has significant effect on the prevention and early treatment of root caries.

关 键 词：精氨酸 变异链球菌 生物膜 牙骨质 再矿化 

分 类 号：R781.1[医药卫生—口腔医学]