锌指蛋白ZNF23在非小细胞肺癌中表达的研究  

The expression of znic finger ZNF23in human non-small cell lung cancer

作　　者：曲微 任洪久 徐艺桐 邱雪杉[2] 赵俊军 QU Wei;REN Hongjiu;XU Yitong;QIU Xueshan;ZHAO Junjun(Department of Pathology,Central Hospital Of Dalian University Of Techology,Dalian116000,China;Department of Pathology,The First Affiliated Hospital of China Medical University,Shenyang110000,China)

机构地区：[1]大连理工大学附属中心医院病理科,辽宁大连116000 [2]中国医科大学附属第一医院病理科,辽宁沈阳110000

出　　处：《中国实验诊断学》2024年第1期57-63,共7页Chinese Journal of Laboratory Diagnosis

摘　　要：目的 探讨ZNF23非小细胞肺癌中的表达、对肺癌生物学行为的影响及相关作用机制。方法 免疫组化检测非小细胞肺癌组织中ZNF23表达,Western Blot及realtime PCR方法检测ZNF23在癌与癌旁组织中蛋白及mRNA表达,siRNA转染及细胞周期实验检测ZNF23在调节细胞周期进展中发挥的作用。结果 与癌旁正常组织相比,ZNF23在非小细胞肺癌临床石蜡切片中表达降低,并与组织类型、分化程度、TNM分期、淋巴结转移相关;Western Blot及realtime PCR结果显示ZNF23的蛋白及mRNA水平在癌旁正常组织中多于癌组织。流式细胞仪结果显示ZNF23干扰细胞周期进展较快,多在S/G2期。结论 ZNF23在非小细胞肺癌组织中的表达低于癌旁正常组织,是一个癌症抑制因子,并且可以通过阻滞细胞周期进展而抑制细胞增殖,从而发挥肿瘤抑制作用。Objective To study the protein expression of ZNF23in lung cancer and its relationgship with clinicopathological.Methods The expression of znic finger ZNF23in human non-small cell lung cancer was tested by immunohistochemistry.ZNF23protein and mRNA expression in NSCLC tissues and normal tissues was examined by western blot and real-time PCR.In addition we also explored the association of ZNF23with cell cycle progression in lung cancer cell lines.Results ZNF23mRNA and protein levels were significantly lower in the tumor tissues compared to the adjacent normal tissues in Non-Small Cell Lung Cancer.There was a close correlation between ZNF23down-regulation and TNM stage,differentiation,histology,tumor status and nodal status.Downregulation of ZNF23by siRNA can induce the cell cycle in S/G2.Conclusion ZNF23is found to be a tumor inhibitor which is downexpressed in Non-Small Cell Lung Cancer.ZNF23inhibits cell growth by inducing cell cycle arrest in Non-Small Cell Lung Cancer cells.

关 键 词：ZNF23 锌指蛋白 细胞周期 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]