小麦酰脲通透酶基因TaUPS2.1-D的克隆及等位变异分析  

Cloning and allelic variation of wheat ureide permease gene TaUPS2.1-D

作　　者：孟晓丹[1] 苏秀荣 程琳 郝世军 张俊辉 李荣荣 任江萍[1] 李磊[1] 王小纯[3] MENG Xiaodan;SU Xiurong;CHENG Lin;HAO Shijun;ZHANG Junhui;LI Rongrong;REN Jiangping;LI Lei;WANG Xiaochun(National Engineering Research Centre for Wheat,Henan Technology Innovation Centre of Wheat,Henan Agricultural University,Zhengzhou 450046,China;College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南省小麦技术创新中心,河南郑州450046 [2]河南农业大学农学院,河南郑州450046 [3]河南农业大学生命科学学院,河南郑州450046

出　　处：《河南农业大学学报》2024年第1期52-58,共7页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(32071956);中国博士后科学基金项目(2022M721045);河南省自然科学基金项目(232300421258);中原学者工作站项目(224400510001)。

摘　　要：【目的】明确小麦酰脲转运蛋白TaUPS2.1-D的亚细胞定位及其在不同小麦品种中的等位变异。【方法】克隆了小麦TaUPS2.1-D基因,构建了TaUPS2.1-D与绿色荧光蛋白(GFP)的融合表达载体,通过转化烟草叶片阐明了TaUPS2.1-D的亚细胞定位。对TaUPS2.1-D基因在几千个小麦品种中的变异位点及其变异频率进行分析。【结果】TaUPS2.1-D基因在细胞质膜和内质网上均有明显表达。TaUPS2.1-D基因的等位变异位点变异频率均较低,可见该基因在不同小麦品种中功能相对保守。【结论】小麦酰脲转运蛋白TaUPS2.1-D是一种跨膜转运蛋白,且功能较为保守。【Objective】To determine the subcellular localization and allelic variation in different wheat varieties of ureide transporter TaUPS2.1-D.【Method】In this study,TaUPS2.1-D gene was cloned in wheat,TaUPS2.1-D was fusion expressed with a green fluorescent protein(GFP)was constructed to clarify the subcellular localization of TaUPS2.1-D by transforming tobacco leaves.Lastly,sites and frequency of allelic variation of TaUPS2.1-D in thousands of wheat varieties were analyzed.【Result】TaUPS2.1-D was obviously expressed in the plasma membrane and endoplasmic reticulum.The variation sites and frequency of allelic variation of TaUPS2.1-D gene is very low,suggesting that its function is relatively conservative in different wheat varieties.【Conclusion】The above studies indicated that ureide transporter TaUPS2.1-D is a transmembrane transporter and the function of TaUPS2.1-D is relatively conservative.

关 键 词：小麦 酰脲通透酶 亚细胞定位 等位变异 氮素利用 

分 类 号：S512.1[农业科学—作物学] Q945.18[生物学—植物学]