盐胁迫下大豆抗感品种根系微生物群落结构分析  

Analysis of root microbiota community structure of soybean resistant and susceptible varieties under salt stress

作　　者：陈昕宇 张真 张早立 宋圆圆 曾任森 卢龙 CHEN Xinyu;ZHANG Zhen;ZHANG Zaoli;SONG Yuanyuan;ZENG Rensen;LU Long(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学农学院,作物遗传育种与综合利用教育部重点实验室,福建福州350002

出　　处：《河南农业大学学报》2024年第1期59-68,共10页Journal of Henan Agricultural University

基　　金：福建省自然科学基金项目(2021J05019);国家自然科学基金项目(32101676)。

摘　　要：【目的】探究盐胁迫下大豆根系微生物群落结构变化及耐盐大豆的根系微生物群落结构特征。【方法】以2种耐盐性不同的大豆品种科丰1(盐敏感)和南农1183-2(耐盐)为试验材料,通过盆栽种植,盐胁迫(120 mmol·L^(-1)NaCl)处理,利用16S扩增子测序技术对其根系微生物组进行高通量测序。【结果】门一级水平上2种大豆优势菌门均为变形菌门Proteobacteria,属水平上不同大豆不同条件下的优势菌属各不相同。盐胁迫条件下,盐敏感品种根系微生物优势菌属组成无较大改变,耐盐品种根系中,根系促生菌(PGPB)假单胞菌属Pseudomonas丰度所占比例明显上升。共现性网络分析表明,盐胁迫条件下变形菌门为维持根系微生物多样性做出了最大贡献。对大豆根系微生物组的功能预测表明,耐盐大豆根际微生物在脂类以及氨基酸代谢途径等方面优于盐敏感大豆。【结论】盐胁迫下2种大豆根系微生物的多样性发生改变,假单胞菌属是盐胁迫条件下耐盐大豆根系微生物的优势菌属,大豆根际的假单胞菌属或可成为盐胁迫下大豆栽培及土壤改良的可利用资源。【Objective】This study aims to investigate the changes in the microbial community structure of soybean roots under salt stress,as well as the characteristics of the root microbial community structure of salt-tolerant soybean.【Method】Two soybean varieties,Kefeng1(salt-sensitive)and Nannong 1183-2(salt-tolerant),with varying levels of salt tolerance as test materials were cultivated in pots and subjected to salt stress(120 mmol·L^(-1)NaCl).The root microbiome of these plants was then analyzed using 16S amplicon sequencing technology.【Result】At the phylum level,Proteobacteria were found to be the dominant bacteria in both groups of soybeans.However,at the genus level,the dominant bacterial genera varied among different soybeans under different conditions.Specifically,under salt stress,there was no significant alteration in the composition of dominant bacterial genera in the roots of salt-sensitive variety.While,the relative abundance of Pseudomonas,a rhizosphere growth-promoting bacteria(PGPB),significantly increased in the roots of salt-tolerant variety.Co-occurrence network analysis revealed that Proteobacteria played a crucial role in maintaining root microbial diversity under salt stress.Functional prediction of the soybean root microbiome indicated that salt-tolerant soybean rhizosphere microorganisms outperformed salt-sensitive soybean in lipid and amino acid metabolic pathways.【Conclusion】The diversity of root microorganisms in both varieties of soybean has been modified in response to salt stress.Pseudomonas emerges as the dominant genus of salt-tolerant soybean root microorganisms under salt stress.Consequently,Pseudomonas in the soybean rhizosphere may serve as valuable resources for soybean cultivation and soil enhancement in salt-stressed conditions.

关 键 词：大豆 盐胁迫 根系微生物 微生物多样性 假单胞菌属 

分 类 号：S565.1[农业科学—作物学]