黄腰胡蜂油脂的抗炎抗氧化活性测试及GC-MS分析  

The anti-inflammatory,antioxidant activity test and GC-MS analysis of oil extracted from Vespa affinis

作　　者：常雅萍 刘超贺 肖怀 张成桂 杨银河 杨大松 CHANG Yaping;LIU Chaohe;XIAO Huai;ZHANG Chenggui;YANG Yinhe;YANG Dasong(Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali University,Dali 671000,China)

机构地区：[1]大理大学云南省昆虫生物医药研发重点实验室,大理671000

出　　处：《生物学杂志》2024年第1期41-45,共5页Journal of Biology

基　　金：云南省科技厅基础研究专项面上项目(202201AT070007,202101AT070031);国家自然科学基金青年科学基金项目(81903924);云南省中青年学术和技术带头人后备人才项目(202105AC160062,202305AC160035);云南省重大科技专项计划项目(202002AA100007)。

摘　　要：研究首先从黄腰胡蜂中提取油脂,并根据极性差异将其划分为3个部位。随后采用DPPH法对其抗氧化活性进行测试,同时采用LPS诱导的小鼠巨噬细胞炎症模型对其体外抗炎活性进行测试。为进一步阐明黄腰胡蜂油脂发挥活性的物质基础,采用GC-MS技术对其油脂的化学成分进行系统分析。结果表明:黄腰胡蜂油脂的抗氧化和抗炎活性成分主要集中在大极性部位;从化学成分来看黄腰胡蜂油脂中富含不饱和脂肪酸,是优质的营养成分。大极性部位在化学成分上含有区别性成分丁香酚和亚麻酸,二者是黄腰胡蜂油脂具有抗氧化和抗炎活性的关键成分。研究不仅丰富了对黄腰胡蜂油脂化学成分和生物活性的认识,还为其进一步现代化开发奠定基础。The oil was extracted from Vespa affinis firstly and divided into three parts based on polarity differences in this work.Its antioxidant activity was then tested by using the DPPH method,while its anti-inflammatory activity was tested in vitro using a mouse macrophage inflammation model induced by LPS.In order to clarify the chemical composition responsible for the activity,a GC-MS analysis of oil extracts were further performed.The results indicated that the antioxidant and anti-inflammatory active components were mainly concentrated in the large polar part.The oil from V.affinis was rich in unsaturated fatty acids,which are high quality nutrients.As the distinguishing and main components in large polar part,eugenol and linolenic acid are key compounds for the antioxidant and anti-inflammatory activity of oil from V.affinis.The completion of this research not only enriched the understanding about the chemical constituent and biological activity of wasp oil,but also laid the foundation for its further development.

关 键 词：黄腰胡蜂 油脂 抗氧化活性 抗炎活性 气相色谱-质谱法 

分 类 号：Q969.554.4[生物学—昆虫学]