氯霉素降解菌Pseudoxanthomonas mexicana CC18的筛选、鉴定和降解特性  

Isolation,identification and degradation characteristics of a chloramphenicol-degrading bacterium,Pseudoxanthomonas mexicana CC18

作　　者：陈田 周佳[1] 汪依萍 耿宁雨 屈建航[1] 赵帅 谷燮 CHEN Tian;ZHOU Jia;WANG Yiping;GENG Ningyu;QU Jianhang;ZHAO Shuai;GU Xie(College of Biological Engineering,Henan University of Technology,Zhengzhou 450000,China)

机构地区：[1]河南工业大学生物工程学院,郑州450000

出　　处：《生物学杂志》2024年第1期94-99,共6页Journal of Biology

基　　金：国家自然科学基金项目(42107139);河南省高等学校重点科研项目(20A180009);河南工业大学青年骨干教师培育计划项目(21421206)。

摘　　要：为挖掘降解氯霉素的微生物菌种资源,从处理氯霉素废水的生物接触氧化反应器内活性污泥中分离筛选得到1株氯霉素降解菌CC18,通过形态观察、生理生化特征、16S rRNA基因序列分析,鉴定菌株CC18归属于墨西哥假黄单胞菌(Pseudoxanthomonas mexicana)。菌株CC18降解氯霉素的最佳条件:接种量4%、pH值为7、培养温度28℃,24 h后其对20 mg/L氯霉素的降解率为22.9%。响应面试验结果表明,当接种量为3.6%,pH值为7.6,温度为30℃时,24 h后菌株CC18对20 mg/L氯霉素的降解率为30.6%。研究发现,墨西哥假黄单胞菌具有降解氯霉素的能力,不仅丰富了氯霉素降解菌库,也为环境中氯霉素污染的微生物强化处理提供了理论基础。In order to explore the microbial strain resources for chloramphenicol degradation,a chloramphenicol-degrading strain CC18 was isolated and screened from the activated sludge in the biological contact oxidation reactor treating chloramphenicol wastewater.The CC18 was identified as Pseudoxanthomonas mexicana based on its morphological,physiological and biochemical characteristics,and 16S rRNA gene sequence analysis.The optimal conditions for degradation chloramphenicol by strain CC18 were 4%inoculum,pH 7,and at incubation temperature 28℃.The degradation efficiency of 20 mg/L chloramphenicol after 24 h was 22.9%.The response surface results further suggested that the degradation efficiency of 20 mg/L chloramphenicol by strain CC18 was 30.6%after 24 h when the inoculum was 3.6%,pH was 7.6,and the temperature was 30℃.The ability of Pseudoxanthomonas mexicana to degrade chloramphenicol was found for the first time in this study,which not only enriched the chloramphenicol degrading bacterial resource,but also provided a theoretical basis for the microbial enhancement treatment of chloramphenicol contamination in the environment.

关 键 词：氯霉素 墨西哥假黄单胞菌 筛选 鉴定 微生物降解 

分 类 号：Q935[生物学—微生物学] X172[环境科学与工程—环境科学]