二次脱钙法在临床病理骨组织HE制片中的应用研究  

Research on the application of secondary decalcification in the HE pathological section of the osseous tissue in clinical pathology

作　　者：李彬 党裔武[1] 邱健峰 陈罡[1] 李奇 黄志广 LI Bin;DANG Yiwu;QIU Jianfeng;CHEN Gang;LI Qi;HUANG Zhiguang(Department of Pathology,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China)

机构地区：[1]广西医科大学第一附属医院病理科,广西南宁530021

出　　处：《现代医药卫生》2024年第3期409-412,共4页Journal of Modern Medicine & Health

摘　　要：目的 探讨二次脱钙法在临床病理骨组织苏木精-伊红(HE)制片中的应用价值。方法 收集2022年6-12月该院病理科脱钙不充分、骨组织块或含钙化组织的蜡块100块,制成病理切片,根据脱钙方法的不同将病理切片分为对照组和研究组,对照组采用常规脱钙法,研究组采用二次脱钙法,分析2种方法制片效果的差异。结果 研究组病理切片HE制片结构完整性、HE染色满意度、切片优良率均明显高于对照组,差异均有统计学意义(P<0.05)。结论 二次脱钙法在临床病理骨组织HE制片中具有较高的应用价值。Objective To explore the application value of secondary decalcification in the hematoxylin-eosin(HE)pathological section of the osseous tissue in clinical pathology.Methods A total of 100 pieces of wax blocks with insufficient decalcification,bone tissue or calcified tissue were collected in this hospital from June 2022 to December 2022.The wax blocks were made into pathological section and divided into the control group and the experimental group according to the different decalcification methods.The control group used the conventional decalcification method,and the experimental group used secondary delcalcifiaction method.Finally,the quality of the two groups of pathological section was compared.Results The structural integrity of the HE pathological section,HE staining satisfaction,and excellent rate of pathological section in the experimental group were all higher than those of the control group.The differences were statistically significant(P<0.05).Conclusion The second ary decalcification method has a high application value in HE pathological section of clinical pathological bone tissue.

关 键 词：二次脱钙 临床病理 骨组织 HE制片 HE染色 

分 类 号：R361.2[医药卫生—病理学] R681[医药卫生—基础医学]