酶辅助法优化金缕梅多酚提取工艺及其美白功效评价的研究  

Enzyme assisted optimization of witch hazel polyphenols extraction process and evaluation of its whitening effect

作　　者：樊远红 杨思敏 李慧婷 秦小桐 吴发亮 彭效明[1] 居瑞军[1] FAN Yuan-hong;YANG Si-min;LI Hui-ting;QIN Xiao-tong;WU Fa-liang;PENG Xiao-ming;JU Rui-jun(College of New Materials and Chemical Engineering,Beijing Institute of Petrochemical Technology,Beijing 102617,China)

机构地区：[1]北京石油化工学院新材料与化工学院,北京102617

出　　处：《现代化工》2024年第2期137-142,共6页Modern Chemical Industry

基　　金：北京市教委科技计划一般项目(KM202210017011,KM201810017002);国家自然科学基金项目(81703453);北京石油化工学院校内学科平台建设项目(2019XK006);北京石油化工学院交叉科研探索项目资助(BIPTCSF-019&021)。

摘　　要：为了优化金缕梅多酚提取工艺及美白功效,采用酶辅助法和响应面法优化金缕梅多酚提取工艺,并检测金缕梅多酚对酪氨酸酶活性及黑色素合成的影响。结果表明,金缕梅多酚提取最优工艺为:纤维素质量分数为2.5%、酶解温度为38℃、酶解时间为85 min、料液比为1∶21、乙醇体积分数为40%,该条件下金缕梅多酚提取率达到62.16 mg/g。金缕梅多酚具有良好的抑制酪氨酸酶活性及黑色素生成的功效,具备潜在的美白功效。The enzyme-assisted method and response surface method are employed to optimize the witch hazel polyphenols extraction process and the whitening effect of polyphenols.The effect of witch hazel polyphenols on the activity of tyrosinase and the synthesis of melanin are detected.It is found that the optimum extraction conditions are as follows:cellulose concentration is 2.5%,enzymolysis temperature is 38℃,enzymolysis time is 85 min,solid-liquid ratio is 1:21,and ethanol concentration is 40%.Under these conditions,the extraction rate of polyphenols reaches 62.16 mg·g-1.Witch hazel polyphenols exhibit good abilities in inhibiting the activity of tyrosinase and blocking melanin synthesis,presenting potential whitening effect.

关 键 词：金缕梅 多酚 酶辅助提取 响应面分析 美白 

分 类 号：R282.4[医药卫生—中药学]