LY294002通过PI3K/Akt通路对结直肠癌RKO细胞增殖及凋亡的影响  

Effect of LY294002 on the proliferation and apoptosis of colorectal cancer RKO cells through PI3K/Akt pathway

作　　者：张恒[1] 李康宁 董海峰 郑英斌[1] Zhang Heng;Li Kangning;Dong Haifeng;Zheng Yingbin(Department of Surgery,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014)

机构地区：[1]郑州大学第二附属医院普通外科,河南郑州450014

出　　处：《中国现代医药杂志》2024年第1期22-27,共6页Modern Medicine Journal of China

摘　　要：目的研究LY294002对结直肠癌(Colorectal cancer,CRC)RKO细胞增殖、凋亡的影响及机制。方法通过免疫组织化学检测p-Akt在CRC癌组织与癌旁组织中的表达情况,培养人CRC RKO细胞,并将其分为3组,分别为空白对照组(Blank组),阴性对照组(DMSO组)和阳性对照组(LY294002组),采用细胞计数(CCK-8)法测定各组细胞增殖能力,分析LY294002对人CRC RKO细胞增殖的影响;采用流式分析检测LY294002对RKO细胞凋亡的影响;Western blot检测各组细胞PI3K/Akt通路中关键分子p-Akt、Akt、Bax和Bcl-2等蛋白的表达差异。结果与癌旁组织相比,CRC组织中p-Akt显著上调。CCK-8结果显示,与其他两组比较,LY294002组RKO细胞存活数减少(P<0.05)。凋亡实验结果显示,与其他两组比较,LY294002组RKO细胞凋亡率显著增加(P<0.05)。Western blot结果显示,与其他两组相比,LY294002组RKO细胞中p-Akt表达明显降低(P<0.05),Bax显著升高(P<0.05)。结论LY294002可以通过PI3K/Akt通路抑制RKO细胞增殖,促进RKO细胞凋亡。Objective To study the effect and mechanism of LY294002 on the proliferation and apoptosis of colorectal cancer RKO cells.Methods The expression of p-Akt in colorectal cancer tissues and adjacent cancer tissues was detected by immuno-histochemistry.Human colorectal cancer RKO cells were cultured and divided into 3 groups:blank group,DMSO group and LY294002 group.Cells’proliferation were determined by CCK-8 assay to analyze the effect of LY294002 on the proliferation of human colorectal cancer RKO cells.The effect of LY294002 on the apoptosis of RKO cells was detected by flow cytometry.Western blot was used to detect the key molecules,such as p-Akt,Akt,Bax and Bcl-2.Results P-Akt was significantly up-regulated in colorectal cancer tissues compared with adjacent cancer tissues.The results of CCK-8 showed that compared with the other two groups,the number of viable cells in LY294002 group was decreased(P<0.05)and the apoptosis rate of LY294002 group was increased significantly(P<0.05).Western blot results showed that compared with the other two groups,the expression of p-Akt in LY294002 group was significantly decreased(P<0.05),and the Bax was significantly increased(P<0.05).Conclusion LY294002 effectively inhibits RKO cells and induces proliferation apoptosis by suppressing the PI3K/Akt pathway.

关 键 词：LY294002 结直肠癌 增殖 凋亡 PI3K/AKT通路 

分 类 号：R735.34[医药卫生—肿瘤]