特勒凯比尔沙门菌临床血流分离株的毒力研究  

Study on the toxicity characteristics of a clinical bloodstream isolate of Salmonella Telelkebir

作　　者：郑恩惠[1] 邱玉锋 陈建辉[1] 高亚东 黄梦颖[1] 李曲文[1] 林杰[1] 翁顺太[1] ZHENG En-hui;QIU Yu-feng;CHEN Jian-hui;GAO Ya-dong;HUANG Meng-ying;LI Qu-wen;LIN Jie;WENG Shun-tai(Fujian Provincial Center for Disease Control and Prevention,Fuzhou 350012,China)

机构地区：[1]福建省疾病预防控制中心,福州350012

出　　处：《中国人兽共患病学报》2024年第1期31-39,共9页Chinese Journal of Zoonoses

基　　金：福建省医学创新项目(No.2020CXB011);福建省创新战略研究项目(No.2022R0087);福建省卫健委青年科研项目(No.2019-2-16)联合资助。

摘　　要：目的构建ICR小鼠感染模型研究特勒凯比尔血清型沙门菌的致病力,对小鼠分离株进行病原学鉴定。方法通过感染小鼠临床症状、组织病理学、组织细菌载量等的变化,观察排菌周期,评价该血清型沙门菌对小鼠的致病性,计算小鼠LD_(50);通过飞行质谱、生化鉴定、血清型鉴定、分子分型等实验,与人分离株进行比对;通过PCR实验、全基因组测序等进行毒力基因分析。结果特勒凯比尔沙门菌LD 50为2.67×10^(8) CFU/mL;感染后0.5 h即可出现临床症状,卷缩、被毛松乱;死亡小鼠解剖发现小肠充血严重,可见较多气泡、积液,盲肠坏死,肝尖点状变性坏死,肾脏表面有坏死灶,脾脏萎缩;小鼠脾脏、肾脏、肺脏、肝脏、空肠细菌载量感染后3 d达到高峰,而心脏第6 d达高峰;高剂量组排菌时间超100 d;组织中CD3含量随剂量增加呈递增状态,炎细胞浸润,心肌毛细血管扩张充血,肝细胞大面积空泡、变性坏死,脾窦扩张明显,分区模糊,肾小球基膜增厚,纤毛上皮部分剥脱,空肠绒毛萎缩、脱落;PCR及全基因组测序发现具有cdtB、pltA、pltB等沙门菌相关毒力基因。结论本研究首次成功建立了特勒凯比尔沙门菌ICR小鼠模型,证明了该血清型沙门菌具有一定的致病力。The ICR(Institute of Cancer Research)mouse infection model was constructed to study the pathogenicity of Salmonella Telelkebir serotype,and the pathogenic identification of mouse isolates was carried out.Observe the bacterial excretion cycle,evaluate the pathogenicity of Salmonella serotype to mice,and calculate the LD 50 by the changes in clinical characteristics,histopathology and tissue bacterial load of infected mice;by flight mass spectrometry,biochemical identification,serotype identification,molecular typing and other experiments,compared with human isolates;virulence gene analysis was carried out by PCR experiment and whole genome sequencing.The LD_(50) of Salmonella Telelkebir is 2.67×10^(8) CFU/mL;curling and fluffing may occur 0.5 h after infection;autopsy of dead mice showed that the small intestine was severely congested,with more bubbles and fluid accumulation,cecal necrosis,liver apical degeneration and necrosis,necrotic foci on the surface of the kidney and spleen atrophy;the bacterial load of spleen,kidney,lung,liver and jejunum in mice reached its peak at 3 days after infection,while that of heart at 6 days;the bacterial excretion time of the high-dose group exceeded 100 days;The level of CD3 in tissues increased with increasing dose,with inflammatory cell infiltration,myocardial capillary dilation and hyperemia,large area of vacuoles,degeneration and necrosis of hepatocytes,obvious enlargement of splenic sinus,blurred zoning,thickening of glomerular basement membrane,partial exfoliation of ciliated epithelium,atrophy and exfoliation of jejunal villi;PCR and whole genome sequencing revealed Salmonella-related virulence genes such as cdtB,pltA and pltB.This study was the first to successfully establish the ICR mouse model of Salmonella Telelkebir,demonstrating that this serotype of Salmonella has some pathogenicity.

关 键 词：特勒凯比尔沙门菌 小鼠模型 病原学鉴定 组织病理学 免疫组化 全基因组序列 

分 类 号：R378.22[医药卫生—病原生物学]