miR-155对慢性髓细胞白血病细胞凋亡及热休克蛋白表达的影响  

Effects of miR-155 on apoptosis of chronic myeloid leukemia cells and expression of heat shock proteins

作　　者：任影丽 陈治东[1] 孙蓓蓓[1] 姚杰[1] 马凡 周强[1] Ren Yingli;Chen Zhidong;Sun Beibei;Yao Jie;Ma Fan;Zhou Qiang(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601

出　　处：《安徽医科大学学报》2024年第1期34-39,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究项目(编号:KJ2021ZD0029);安徽医科大学校科研基金(编号:2022xkj039)。

摘　　要：目的探讨微小RNA-155(miR-155)对慢性髓细胞白血病(CML)细胞凋亡情况和对热休克蛋白(HSP)27、HSP60及HSP70表达的影响。方法采用实时荧光定量逆转录PCR(RT-qPCR)分别检测miR-155在CML耐伊马替尼(IM)细胞株K562-G和CML细胞株K562中的表达水平。以携带miR-155的慢病毒和阴性对照慢病毒感染K562-G细胞,分别命名为miR-155组和对照组。细胞计数试剂盒8(CCK-8)检测miR-155对耐药细胞增殖的影响。RT-qPCR及蛋白质印迹法(Western blot)检测miR-155对HSP27、HSP60、HSP70表达的影响。流式细胞术检测miR-155组和对照组细胞的凋亡比例。结果与K562细胞相比,K562-G细胞中miR-155呈低表达(P<0.05)。miR-155组细胞的增殖情况与对照组相比从第36 h开始降低(P<0.05)。与对照组相比,miR-155组HSP60、HSP70升高(P<0.05),而HSP27降低(P<0.01)。流式细胞术检测结果显示,miR-155组细胞的凋亡率高于其对照组(P<0.05)。结论miR-155促进CML细胞的凋亡,并使细胞中的HSP60、HSP70表达升高,HSP27表达降低。Objective To explore the effects of microRNA-155(miR-155)on apoptosis of chronic myeloid leuke-mia(CML)cells,and the influence of miRNA-155 regulating the expression of heat shock proteins(HSP)27,HSP60,HSP70.Methods Reverse transcription-quantitative real-time PCR(RT-qPCR)was used to detect the expression levels of miR-155 in CML-resistant imatinib(IM)cell line K562-G and CML cell line K562.K562-G cells were infected with the lentivirus carrying miR-155 or the negative control lentivirus,and they were named miR-155 group and control group.The effect of miR-155 on the proliferation of drug-resistant cells was detected by cell counting kit-8(CCK-8)method.RT-qPCR and Western blot were used to detect the effect of miR-155 on the expression of heat shock proteins HSP27,HSP60,HSP70.Flow cytometry was used to detect the percentage of cell apoptosis in miR-155 group and control group.Results Compared with K562 cells,miR-155 showed low expression in K562-G cells(P<0.05).The proliferation of miR-155 group cells decreased significantly from the 36th hour(P<0.05).Compared with the control group,in the miR-155 group,HSP60 and HSP70 increased(P<0.05),while HSP27 decreased(P<0.01).The apoptosis rate of miR-155 group was higher than that of control group(P<0.05).Conclusion miR-155 promotes the apoptosis of chronic myeloid leukemia cells,increases the expression of HSP60 and HSP70,and decreases the expression of HSP27.

关 键 词：MIR-155 慢性髓细胞白血病 耐药 热休克蛋白 细胞增殖 凋亡 

分 类 号：R773.72[医药卫生—眼科]