血清lncRNA HOTAIR、miR-197-3p水平与精神分裂症患者精神症状及认知功能的相关性  

The correlation between serum lncRNA HOTAIR,miR-197-3p levels and psychiatric symptoms and cognitive function in patients with schizophrenia

作　　者：黄伟杰 李一兰 王西林 卢林生 肖攀攀[1] Huang Weijie;Li Yilan;Wang Xilin;Lu Linsheng;Xiao Panpan(Department of Psychiatry,The Affiliated Brain Hospital of Guangzhou Medical University,Guangdong Province,Guangzhou 510370,China)

机构地区：[1]广州医科大学附属脑科医院精神科,广州510370

出　　处：《疑难病杂志》2024年第2期175-180,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：2022年度广东省医学科研基金项目(B2022290)。

摘　　要：目的探讨血清长链非编码RNA-HOX转录本反义基因间RNA(lncRNA HOTAIR)、微小RNA-197-3p(miR-197-3p)水平与精神分裂症(SCZ)患者精神症状及认知功能的相关性。方法选取2021年4月—2023年3月广州医科大学附属脑科医院精神科诊治SCZ患者118例为研究对象(SCZ组),并选取同期健康体检者110例作为健康对照组。实时荧光定量PCR(qRT-PCR)检测血清lncRNA HOTAIR、miR-197-3p水平,对参与者进行阳性与阴性症状量表(PANSS)评分和认知功能评价;Pearson、Spearman相关系数分析血清lncRNA HOTAIR与miR-197-3p水平及二者与精神症状、认知功能的相关性;受试者工作特征(ROC)曲线分析血清lncRNA HOTAIR、miR-197-3p水平对SCZ的评估价值。结果与健康对照组比较,SCZ组血清lncRNA HOTAIR表达水平升高,miR-197-3p水平降低(t/P=9.859/<0.001、19.191/<0.001)。生物信息学预测,lncRNA HOTAIR与miR-197-3p存在结合位点。Pearson相关性分析结果显示,SCZ患者血清中lncRNA HOTAIR表达与miR-197-3p表达呈负相关(r=-0.543,P<0.001)。与健康对照组比较,SCZ组阳性症状、阴性症状、一般病理症状及总分均显著升高(t/P=31.623/<0.001、28.219/<0.001、48.918/<0.001、39.574/<0.001),TMT、BACS、WMS-III-SS、BVMT、HVLT、CPT以及SCWT中单次测验、颜色测验评分显著降低(t/P=6.520/<0.001、7.666/<0.001、4.114/<0.001、8.191/<0.001、5.902/<0.001、4.985/<0.001、13.060/<0.001、8.938/<0.001)。SCZ患者lncRNA HOTAIR水平与阴性症状、总分呈正相关(r/P=0.498/<0.001、0.507/<0.001),与TMT、CPT呈负相关(r/P=-0.476/<0.001、-0.485/<0.001);miR-197-3p与阴性症状、总分呈负相关(r/P=-0.408/<0.001、-0.453/0.009),与TMT、CPT呈正相关(r/P=0.449/0.001、0.517/<0.001)。血清lncRNA HOTAIR、miR-197-3p及二者联合预测SCZ发生的AUC分别为0.819、0.885、0.927,二者联合预测AUC显著高于各自单独预测(Z/P=4.580/<0.001、2.953/0.003)。结论SCZ患者体内血清lncRNA HOTAIR上调,miR-197-3p下调,与精神症状�Objective To investigate the correlation between serum long chain non coding RNA-HOX transcript antisense interRNA(lncRNA HOTAIR)and microRNA-197-3p(miR-197-3p)levels with psychiatric symptoms and cognitive function in patients with schizophrenia(SCZ).Methods One hundred and eighteen patients with SCZ treated in the Department of Psychiatry of Guangzhou Medical University Affiliated Brain Hospital from April 2021 to March 2023 were selected as the study subjects(SCZ group),and 110 healthy individuals who underwent physical examinations during the same period were selected as the healthy control group.Real time fluorescence quantitative PCR(qRT PCR)was used to detect serum levels of lncRNA HOTAIR and miR-197-3p,and participants were evaluated for positive and negative symptom scale(PANSS)scores and cognitive function;Pearson Spearman correlation coefficient analysis of serum lncRNA HOTAIR and miR-197-3p levels,as well as their correlation with psychiatric symptoms and cognitive function;The evaluation value of receiver operating characteristic(ROC)curve analysis of serum lncRNA HOTAIR and miR-197-3p levels for SCZ.Results Compared with the healthy control group,the serum lncRNA HOTAIR expression level in the SCZ group increased,while the miR-197-3p level decreased(t/P=9.859/<0.001,19.191/<0.001).Bioinformatics predicts that there is a binding site between lncRNA HOTAIR and miR-197-3p.The Pearson correlation analysis results showed a negative correlation between lncRNA HOTAIR expression and miR-197-3p expression in the serum of SCZ patients(r=-0.543,P<0.001).Compared with the healthy control group,the positive symptoms,negative symptoms,general pathological symptoms,and total score of the SCZ group were significantly increased(t/P=31.623/<0.001,28.219/<0.001,48.918/<0.001,39.574/<0.001).TMT,BACS,WMS-III-SS,BVMT,HVLT,CPT,and SCWT were tested in a single test The color test score significantly decreased(t/P=6.520/<0.001,7.666/<0.001,4.114/<0.001,8.191/<0.001,5.902/<0.001,4.985/<0.001,13.060/<0.001,8.938/<0.001).The l

关 键 词：精神分裂症 精神症状 认知功能 长链非编码RNA-HOX转录本反义基因间RNA 微小RNA-197-3p 

分 类 号：R749.3[医药卫生—神经病学与精神病学]