利清颗粒辅助降低大鼠血尿酸作用及安全性研究  

Effect and safety of Liqing granules in assisting to reduce serum uric acid in rats

作　　者：胡培丽[1] 郑济凡 刘师卜[1] 张露勇[1] 刘婷[1] 马利波 张励 李波[1] HU Peili;ZHENG Jifan;LIU Shibo;ZHANG Luyong;LIU Ting;MA Libo;ZHANG Li;LI Bo(China Institute for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《上海预防医学》2023年第12期1253-1257,共5页Shanghai Journal of Preventive Medicine

基　　金：国家重点研发计划(2018YFC1706800)。

摘　　要：【目的】建立高尿酸血症(HUA)大鼠模型,研究利清颗粒辅助降血尿酸作用并对其安全性进行评价。【方法】将雄性SD大鼠按体重随机分为溶剂对照组和模型组,模型组灌胃氧嗪酸钾造模7 d后检测血清尿酸(SUA)。根据SUA水平随机分为模型对照组、阳性对照组和低中高剂量组。模型组各组上午继续灌胃氧嗪酸钾,下午分别给予0.5%羧甲基纤维素钠(CMC‑Na)、10 mg·kg^(-1)苯溴马隆(按体重计)和利清颗粒0.6、1.2、2.4 g·kg^(-1)(按体重计)。溶剂对照组上、下午均给予同体积0.5%CMC‑Na混悬液。给予受试物32、45 d后,检测大鼠SUA、肌酐(CREA)、丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)水平。【结果】模型组造模7 d后SUA为(218±23)μmol·L^(-1),较溶剂对照组显著升高(P<0.001)。给予受试物32 d后各剂量组SUA未见降低(P>0.05);中、高剂量组CREA显著降低(P<0.05)。给予受试物45 d后各剂量组SUA均显著降低(P<0.01),CREA、ALT及AST较模型对照组差异均无统计学意义(P>0.05)。【结论】利清颗粒对HUA模型大鼠具有辅助降血尿酸作用,未见相关肝肾功能损伤。[Objective]To establish a rat model of hyperuricemia(HUA),to study the effect of Liqing granules on lowering serum uric acid,and to evaluate its safety.[Methods]Male SD rats were randomly divided into solvent control group and model group according to their body weight.For the model group,serum uric acid(SUA)was determined after 7 days of intra-gastric administration of potassium oxyazinate.The model group were randomly divided into model control group,positive control group,and low,medium,high dose group based on SUA level.Each group from the model group continued to receive potassium oxyazinate in the morning.The animals in the model groups received 0.5%CMCNa,10 mg·kg^(−1) benzbromarone(Doses by body weight)and Liqing granules 0.6,1.2,2.4 g·kg^(−1)(Doses by body weight),respectively in the afternoon.0.5%CMC-Na suspension with the same volume was given both in the morning and afternoon for the solvent control group.Levels of SUA,creatinine(CREA),alanine aminotransferase(ALT)and aspartate transaminase(AST)were determined after 32 and 45 days administration of the test substance.[Results]SUA of the model group was(218±23)μmol·L−1 after 7 days of modeling,which was significantly higher than that of the solvent control group(P<0.001).After 32 days administration of the test substance,SUA didn’t significantly decrease in each dose group(P>0.05).CREA in the medium and high dose groups significantly decreased(P<0.05).After 45 days administration of the test substance,SUA in each dose group was significantly decreased(P<0.001),but CREA,ALT,and AST were not significantly different in each dose group in comparison with the model control group(P>0.05).[Conclusion]Liqing granules can assist in lowering blood serum uric acid in the rat HUA model,and no damage to liver and kidney function is found.

关 键 词：高尿酸血症 利清颗粒 氧嗪酸钾 苯溴马隆 大鼠 肝功能 肾功能 

分 类 号：R115[医药卫生—公共卫生与预防医学]