家蚕BmAKR基因家族的鉴定及在蚕卵中的表达分析  

Genome-wide identification of the BmAKR gene family in the silkworm(Bombyx mori)and their expression analysis in diapause eggs and nondiapause eggs

作　　者：龚竞[1,2] 张伟[1,2] 王清浪 朱子健 庞家欣 侯勇[1,3] GONG Jing;ZHANG Wei;WANG Qinglang;ZHU Zijian;PANG Jiaxin;HOU Yong(State Key Laboratory of Resource Insects,Southwest University,Chongqing 400715,China;College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing 400715,China;Biological Science Research Center,Academy for Advanced Interdisciplinary Studies,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学资源昆虫高效养殖与利用全国重点实验室,重庆400715 [2]西南大学蚕桑纺织与生物质科学学院,重庆400715 [3]西南大学前沿交叉学科研究院生物学研究中心,重庆400715

出　　处：《生物工程学报》2023年第12期4982-4995,共14页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2022YFD1201600);国家自然科学基金(31872429);重庆市自然科学基金(cstc2021jcyj-msxm X1166)。

摘　　要：醛酮还原酶超家族(aldo-keto reductase super family,AKRs)具有广泛的底物特异性,目前尚未见对昆虫AKR基因家族成员的系统鉴定报道。本研究采用生物信息学手段,预测了家蚕(Bombyx mori)AKR基因的系统进化、理化性质、染色体定位、保守基序和基因结构,通过转录组数据和实时荧光定量聚合酶链式反应(quantitative real time polymerase chain reaction,q RT-PCR)分析了不同组织时期及不同发育状态蚕卵中Bm AKR基因的表达水平,并采用Western blotting检测了蚕卵中该蛋白的表达量。本研究共鉴定出11个Bm AKR基因,分布在4条不同的染色体上,都具有AKR家族特有的(α/β)8桶保守结构,理化特征较为相似;系统进化分析显示,其可分为2个家族(AKR1和AKR2)。转录组数据分析显示,家族成员基因在不同组织时期中的表达差异较大。进一步分析发现,一部分Bm AKR基因在非滞育卵的表达量显著高于滞育卵;但Bm AKR1-1在滞育卵中的表达量却显著高于非滞育卵。蛋白水平的检测发现Bm AKR1-1在滞育卵和非滞育卵的差异变化趋势与q RT-PCR结果一致。综上所述,本研究通过对家蚕Bm AKR家族的鉴定和分析,筛选出Bm AKR1-1等作为调控蚕卵发育的候选基因,以便后期对其进行深入研究。The aldo-keto reductase super family(AKRs)has a wide range of substrate specificity.However,the systematic identification of insect AKR gene family members has not been reported.In this study,bioinformatics methods were used to predict the phylogenetic evolution,physical and chemical properties,chromosome location,conserved motifs,and gene structure of AKR family members in Bombyx mori(BmAKR).Transcriptome data or quantitative real time polymerase chain reaction(qRT-PCR)were used to analyze the expression level of BmAKR genes during different organizational periods and silkworm eggs in different developmental states.Moreover,Western blotting was used to detect the expression level of the BmAKR in silkworm eggs.The results showed that 11 BmAKR genes were identified.These genes were distributed on 4 chromosomes of the silkworm genome,all of which had the(α/β)8-barrel motif,and their physical and chemical characteristics were relatively similar.Phylogenetic analysis showed that the BmAKR genes could be divided into 2 subgroups(AKR1 and AKR2).Transcriptome data analysis showed that the expression of BmAKR genes were quite different in different tissues and periods.Moreover,the expression analysis of BmAKR genes in silkworm eggs showed that some genes were expressed significantly higher in nondiapause eggs than in diapause eggs;but another gene,BmAKR1-1,was expressed significantly higher in diapause eggs than in nondiapause eggs.The detection of protein level found that the difference trend of BmAKR1-1 in diapause eggs and non-diapause eggs was consistent with the results of qRT-PCR.In conclusion,BmAKR1-1 was screened out as candidates through the identification and analysis of the BmAKR genes in silkworm,which may regulate silkworm egg development is worthy of further investigation.

关 键 词：家蚕 BmAKR基因家族 滞育卵 表达分析 系统进化 

分 类 号：S881[农业科学—特种经济动物饲养]