沙棘中黄酮类化合物提取及抗氧化活性  

Extraction and antioxidant activity of flavonoids from sea-buckthorn

作　　者：吴翠芳 刘雅宣 李宇辉[3] 郝奇奇 何旭豪 王俊钢 WU Cuifang;LIU Yaxuan;LI Yuhui;HAO Qiqi;HE Xuhao;WANG Jungang(Biology and Food Engineering Department,Bozhou University,Bozhou 236800,China;Bozhou Natural Products Separation and Purification Engineering Technology Research Center,Bozhou 236800,China;Institute of Agro-products Processing Science and Technology,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China)

机构地区：[1]亳州学院生物与食品工程系,安徽亳州236800 [2]亳州市天然产物分离纯化工程技术研究中心,安徽亳州236800 [3]新疆农垦科学院农产品加工研究所,新疆石河子832000

出　　处：《安徽科技学院学报》2024年第1期88-96,共9页Journal of Anhui Science and Technology University

基　　金：国家自然科学基金(31860437);安徽省大学生创新创业训练计划项目(S202312926034);食品科学与工程安徽省高峰培育学科项目;亳州学院一流学科项目(BYXKA202202)。

摘　　要：目的:优化沙棘中总黄酮提取工艺,明确其抗氧化性能。方法:以沙棘为研究对象,以沙棘黄酮得率为指标值,使用乙醇溶液作为提取剂,采用超声波辅助萃取法,对提取时间(A)、提取温度(B)、乙醇体积分数(V乙醇/V总)(C)、料液比(D)进行单因素试验和响应面分析,考察4个因素对总黄酮得率的影响,并对沙棘黄酮的体外抗氧化活性进行检测。结果:在提取时间23 min、提取温度80℃、50%乙醇、料液比1∶20条件下沙棘黄酮得率最高,为2.316 mg/g。各因素对沙棘黄酮得率的影响大小依次为:料液比、乙醇体积分数、提取时间、提取温度。体外抗氧化结果表明,沙棘黄酮具有很好的DPPH自由基和羟自由基清除能力,当沙棘黄酮粗提取物质量浓度达到5 mg/mL时,沙棘黄酮对DPPH自由基的清除率达87.5%,对羟自由基的清除率达到了77.7%。结论:沙棘中含有丰富的黄酮类物质,且具有较好的抗氧化性能。Objective:In order to optimize the extraction process of total flavonoids in sea-buckthorn and clarify its antioxidant property,sea-buckthorn was selected as the research object in this experiment.Methods:The yield of sea-buckthorn flavonoids was used as the index value,and ethanol solution was used as the extractant and combined with ultrasonic-assisted extraction technology,single factor test and response surface method were carried out on the four factors of extraction time,extraction temperature,ethanol concentration and solid-liquid ratio,and the antioxidant property of sea-buckthorn flavonoids were studied.Results:The optimum extraction conditions were as follows:extraction time 23 min,extraction temperature 80℃ethanol concentration 50%,solid-liquid ratio 1∶20 g/mL.Under the conditions,the yield of sea-buckthorn flavonoids was the highest,which was 2.316 mg/g.The effects of various factors on the yield of flavonoids were solid-liquid ratio,ethanol concentration,extraction time and extraction temperature.The antioxidant results showed that sea-buckthorn flavonoids had excellent scavenging effects on DPPH free radicals and hydroxyl radicals.When the concentration of sea-buckthorn flavonoids reached 5 mg/mL,the scavenging rate of DPPH free radical reached 87.5%,and the scavenging rate of hydroxyl radicals reached 77.7%.Conclusion:Sea-buckthorn is rich in flavonoids,and under certain concentration conditions,sea-buckthorn flavonoids have good antioxidant properties.

关 键 词：沙棘 黄酮 提取工艺 响应面法 抗氧化 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]