广泛靶向代谢组学分析不同采收期紫黑香米和普通大米成分差异  

Widely Targeted Metabolomics Analysis of Compositional Differences between Purple Black Rice and Common Rice at Different Harvest Periods

作　　者：田程飘 龙凌云[1] 刘功德[1] 叶雪英[1] 谢秀萍 檀小辉[1] 王丽萍[1] TIAN Chengpiao;LONG Lingyun;LIU Gongde;YE Xueying;XIE Xiuping;TAN Xiaohui;WANG Liping(Guangxi Subtropical Crops Research Institute,Nanning 530001,China;Guangxi Rongshui Yuanbaoshan Miorun Specialty Wine Co.Ltd.,Liuzhou 545300,China)

机构地区：[1]广西壮族自治区亚热带作物研究所,广西南宁530001 [2]广西融水元宝山苗润特色酒业有限公司,广西柳州545300

出　　处：《食品科学》2024年第1期125-132,共8页Food Science

基　　金：广西自然科学基金项目(2021GXNSFAA220084);广西农业科学院基本科研业务专项(桂农科2021YT143)。

摘　　要：采用超高效液相色谱-串联质谱联用广泛靶向代谢组学对广西地区产的紫黑香米和普通大米进行分析,主要分析紫黑香米与普通大米代谢物种类及含量差异,并阐述显著差异代谢途径。样品中共鉴定出667个代谢物,包含脂质、类黄酮、酚酸、生物碱及有机酸等几大类;通过主成分分析、聚类分析及整体代谢物剖析发现,紫黑香米与普通大米相比,成熟样品组间存在的278种差异代谢物中上调的有239种,提前20d采收组存在的267种差异代谢物中上调的有235种;不同时期的紫黑香米成分相似,说明提前采收的紫黑香米兼具食用粥软糯口感及完整营养结构。京都基因与基因组百科全书分析发现与普通大米相比,在紫黑香米中显著上调的鼠李素-3-O-葡萄糖苷、橙皮素-7-O-葡萄糖苷、高圣草酚、圣草酚、橙皮素、儿茶素、槲皮素、柚皮素查耳酮等化合物参与到黄酮和黄酮醇的生物合成途径,调节黄酮、黄酮醇类物质含量,提升紫黑香米营养品质及抗氧化功效。In this study,widely targeted metabolomics based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to analyze the types and contents of differential metabolites between purple black rice and common rice produced in Guangxi and to illustrate the significantly differential metabolic pathways.A total of 667 metabolites were identified,including lipids,flavonoids,phenolic acids,alkaloids,and organic acids.Principal component analysis(PCA),cluster analysis and overall metabolite profiling showed that 239 of the 278 differential metabolites identified in mature rice samples were up-regulated,and 235 of the 267 differential metabolites identified in the 20 day earlier harvest group were up-regulated when compared with common rice.The metabolite composition of purple black rice at different harvest periods was similar,indicating that porridge made from purple black rice harvested in advance will have soft and glutinous texture as well as complete nutritional structure.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that significantly up-regulated compounds such as rhamnose-3-O-glucoside,hesperidin-7-O-glucoside,homoeriodictyol,eriodictyol,hesperidin,catechin,quercetin,naringin and chalcones in purple black rice were involved in the biosynthetic pathways of flavonoids and flavonols,regulating the contents of flavonoids and flavonols,and improving the nutritional quality and antioxidant effect of purple black rice.

关 键 词：紫黑香米 普通大米 广泛靶向代谢组学 差异代谢物 代谢途径 

分 类 号：TS213.3[轻工技术与工程—粮食、油脂及植物蛋白工程]