转录因子CEBPB激活FJX1促进结肠癌细胞的增殖、侵袭、迁移和血管生成能力的研究  

The research of transcription factor CEBPB activates FJX1 to promote the proliferation,invasion,migration and angiogenesis of colon cancer cells

作　　者：滕向龙 朱锡元[1] 邹武军[1] TENG Xiangong;ZHU Xiyuan;ZOU Wujun(Department of Anorectal Surgery,Lishui People’s Hospital,Lishui 323000,Zhejiang,China)

机构地区：[1]丽水市人民医院肛肠外科,浙江丽水323000

出　　处：《中国现代医生》2024年第3期35-41,共7页China Modern Doctor

基　　金：2022年度丽水市本级公益性技术应用研究计划项目(2022GYX32)。

摘　　要：目的 探究CCAAT增强子结合蛋白B(CCAATenhancerbindingproteinbeta,CEBPB)与四连接同源基因(four-jointed box kinase 1,FJX1)在结肠癌(colon cancer,CC)中的调控关系及其对CC恶性进展及血管生成的影响。方法 通过生物信息学分析FJX1和CEBPB在CC中的表达情况及二者之间的调控关系。利用实时荧光定量核酸扩增检测系统(real-time quantitative reverse transcription polymerase chain reaction,qRT-PCR)验证FJX1与CEBPB在CC细胞中的表达情况,利用染色质免疫沉淀(chromatin immunoprecipitation,CHIP)与双荧光素酶实验验证FJX1与CEBPB之间的结合关系。利用细胞计数试剂-8(cellcountingkit-8,CCK-8)、划痕试验、Transwell和血管形成实验检测FJX1与CEBPB对CC细胞增殖、迁移、侵袭及血管形成能力的影响。结果 本研究发现FJX1在CC中高表达,抑制FJX1的表达会显著抑制结肠癌细胞的细胞增殖、迁移、侵袭及血管形成能力。CEBPB是FJX1的上游调控基因,且CEBPB在结肠癌中高表达。CHIP与双荧光素酶实验结果显示,CEBPB可与FJX1相结合。细胞实验结果表明,转录因子CEBPB可通过激活FJX1的表达,从而促进CC细胞的增殖、迁移、侵袭及血管生成。结论 CEBPB/FJX1轴在CC的进展中发挥促癌作用,提示CEBPB和FJX1可能是CC的潜在治疗靶点。Objective To explore the regulatory relationship between CCAAT enhancer binding protein beta(CEBPB)and four-jointed box kinase 1(FJX1)in colon cancer and their effect on colon cancer(CC)malignant progression and angiogenesis.Methods Bioinformatics was used to analyze the expression of FJX1 and CEBPB in CC and the regulatory relationship between them.Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to verify the expression of FJX1 and CEBPB in CC cells,and chromatin immunoprecipitation(CHIP)and dual luciferase assay were used to verify the binding relationship between FJX1 and CEBPB.The effects of FJX1 and CEBPB on the viability,migration,invasion and angiogenesis of CC cells were detected by cell counting kit-8(CCK-8),scratch test,Transwell and angiogenesis test.Results This study revealed that FJX1 was highly expressed in CC.Inhibiting the expression of FJX1 could significantly inhibit the cell viability,migration,invasion and angiogenesis of CC cells.Subsequently,we found that CEBPB was an upstream regulatory gene of FJX1,and CEBPB was highly expressed in CC.CHIP and dual luciferase experiments showed that CEBPB could bind to FJX1.The results of cell experiments showed that the transcription factor CEBPB could promote the proliferation,migration,invasion and angiogenesis of CC cells by activating FJX1.Conclusion CEBPB/FJX1 axis played a cancer-promoting role in the progression of CC,suggesting that CEBPB and FJX1 may be potential therapeutic targets for CC.

关 键 词：结肠癌 CCAAT增强子结合蛋白B 四连接同源基因 血管生成 细胞增殖 

分 类 号：R735[医药卫生—肿瘤]