高尔基运输1A蛋白促进甲状腺癌细胞的增殖和转移  

Golgi Transport 1A Promotes Cell Proliferation and Metastasis of Papillary Thyroid Carcinoma

作　　者：柯淑红[1] 徐朱俊[2] 周杨[1] 郑承红[1] KE Shuhong;XU Zhujun;ZHOU Yang;ZHENG Chenghong(Department of Endocrinology,Wuhan No.1 Hospital,Wuhan 430030,China;Department of Oncology,WuhanNo.1 Hospital,Wuhan 430030,China)

机构地区：[1]武汉市第一医院内分泌科,湖北武汉430030 [2]武汉市第一医院肿瘤科,湖北武汉430030

出　　处：《中山大学学报（医学科学版）》2024年第1期69-75,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：湖北省自然科学基金(2011CDB300)。

摘　　要：【目的】探讨高尔基运输1A蛋白(GOLT1A)在甲状腺癌细胞中的表达水平及其对甲状腺癌细胞增殖、迁移、侵袭和上皮-间充质转化(EMT)的影响。【方法】利用在线数据库肿瘤免疫评估资源(TIMER)、阿拉巴马大学伯明翰分校癌症数据分析(UALCAN)和表达谱交互分析2(GEPIA2)在线分析GOLT1A在甲状腺癌织中的表达。采用实时荧光定量PCR方法和Western blot检测人甲状腺癌细胞中GOLT1A的表达水平,CCK-8实验、集落形成实验和Transwell实验检测GOLT1A表达对人甲状腺癌细胞增殖、迁移和侵袭能力的影响,Western blot检测EMT相关基因E-钙黏蛋白(E-cadherin)、波形蛋白(vimentin)和N-钙黏蛋白(N-cadherin)表达的影响。【结果】TIMER、UAL⁃CAN及GEPIA2在线数据库分析显示,与甲状腺癌癌旁正常组织相比,GOLT1A在甲状腺癌组织中表达升高;qPCR和Western blot结果显示,与正常对照细胞相比,GOLT1A在甲状腺癌细胞系中的表达显著上调。敲低人甲状腺乳头状癌细胞TCP1细胞GOLT1A表达后,抑制细胞增殖、细胞迁移侵袭。敲低GOLT1A后TCP1细胞E-cadherin表达升高,N-cadherin和vimentin表达降低。过表达人甲状腺乳头状癌细胞BCPAP细胞GOLT1A,促进细胞增殖、迁移和侵袭。过表达GOLT1A后BCPAP细胞E-cadherin表达降低,N-cadherin和vimentin表达增加。【结论】GOLT1A在甲状腺癌中高表达,可促进甲状腺癌细胞的增殖、迁移和侵袭。【Objective】To investigate the expression level of Golgi transport 1A(GOLT1A)in thyroid carcinoma and its effects on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of thyroid carcinoma cells.【Methods】The expression of GOLT1A in thyroid carcinoma was analyzed online by tumor immune estimation resource(TIMER),the University of Alabama at Birmingham cancer data analysis portal(UALCAN),gene expression profiling interactive analysis 2(GEPIA2).The expression level of GOLT1A in thyroid carcinoma cells was detected by real�time fluorescence quantitative polymerase chain reaction(RTFQ-PCR)and western blot.Cell counting kit-8(CCK-8)assay,colony formation assay,and transwell assay were used to detect the effects of GOLT1A expression on the proliferation,migration,and invasion of thyroid carcinoma cells.Western blot assay was used to detect the effect of GOLT1A on the expression of EMT-related genes including E-cadherin,vimentin,and N-cadherin.【Results】The online analysis of GEPIA2,TIMER,and UALCAN showed that the expression of GOLT1A was higher in thyroid carcinoma than in normal tissues,and the expression of GOLT1A in thyroid carcinoma cells was significantly higher than in normal control cells.Knockdown of GOLT1A inhibited TPC1 cell proliferation,migration,and invasion.The expression of E-cadherin increased and the expressions of N-cadherin and vimentin decreased in GOLT1A knockdown TPC1 cells.Overexpression of GOLT1A promoted BCPAP cell proliferation,migration,and invasion.The expression of E-cadherin decreased and the expressions of N-cadherin and vimentin increased in GOLT1A overexpression BCPAP cells.【Conclusion】GOLT1A is highly expressed in thyroid carcinoma and can promote the proliferation,migration,and invasion of thyroid carcinoma cells.

关 键 词：甲状腺癌 高尔基运输1A蛋白 增殖 迁移 侵袭 

分 类 号：R736.1[医药卫生—肿瘤] R329.28[医药卫生—临床医学] R730.21