基于转录组数据的绢毛委陵菜MYB转录因子家族分析  

Analysis of the MYB Transcription Factor Family of Potentilla sericata Based on Transcriptome Data

作　　者：刘振[1] 武俊刚[1] 李响 夏威 秦建 刘庆鹏 王学刚 范卫芳 吴建慧[2] Liu Zhen;Wu Jungang;Li Xiang;Xia Wei;Qin Jian;Liu Qingpeng;Wang Xuegang;Fan Weifang;Wu Jianhui(Longjian Road&Bridge Co.,Ltd.,Harbin,150000;College of Landscape Architecture,Northeast Forestry University,Harbin,150000)

机构地区：[1]龙建路桥股份有限公司,哈尔滨150000 [2]东北林业大学园林学院,哈尔滨150000

出　　处：《分子植物育种》2024年第3期751-760,共10页Molecular Plant Breeding

基　　金：七台河市山水林田湖草桃山区域生态环境治理项目EPC工程(6101020201)资助。

摘　　要：利用生物信息学方法,从绢毛委陵菜转录组数据中筛选出63个MYB转录因子,分析了它们的转录因子家族序列特征、理化性质、保守结构域、亚细胞定位和进化关系。结果表明,筛选出的63个绢毛委陵菜转录因子,其中1R-MYB类22个、R2R3-MYB类37个、3R-MYB类3个、4R-MYB类1个。结构域分析表明其具有典型的W型R2R3保守基序;绢毛委陵菜的所有MYB基因都是亲水性不稳定蛋白;而亚细胞定位预测都位于细胞核内;系统进化分析将绢毛委陵菜R2R3-MYB类蛋白分为22个亚家族,其中参与调控重金属镉相关的拟南芥同源基因主要属于S20亚族。研究结果为深入探究绢毛委陵菜MYB基因在调控重金属镉过程中发挥的功能提供基础资料。Sixty-three MYB transcription factors were extracted from the Potentilla sericea transcriptome database by bioinformatics methods,and there was a comprehensive analysis of the sequence characteristics,physical and chemical properties,conserved domains,evolutionary relationships and cell locationat the same time which aim to screen out those genes that responses to Cd stress.The results showed that 63 MYB genes were identified in Potentilla sericea,37 of them belonged to R2R3-MYB,22 of them belonged to 1R-MYB,3 of them belonged to 3R-MYB and 1 of them belonged to 4R-MYB.Furthermore structural domain analysis showed that they have typical W type R2R3 conservative motifs;in the mean time,all those MYB proteins of Potentilla sericea were unstably hydrophilic proteins;subcellular localization prediction showed that MYB proteins of Potentilla sericea were all localized in nucleus.The phylogenetic of MYB proteins of Potentilla sericea were divided into 22 subfamilies according to the classification method of Arabidopsis family.The regulating genes related to heavy metal cadmium belong to the S20 subfamilies.This study showed that MYB genes of Potentilla sericea play an important regulatory role in responses to heavy metal cadmium and providing basic information for further exploration of the function of MYB genes of Potentilla sericea.

关 键 词：绢毛委陵菜 MYB转录因子 生物学分析 镉胁迫 

分 类 号：X173[环境科学与工程—环境科学] Q943.2[生物学—植物学]