Principles of amino-acid–ribonucleotide interaction revealed by binding affinities between homogeneous oligopeptides and singlestranded RNA molecules  

作　　者：Pengyu Wang Xiaocui Fang Ping Li Minxian Li Yanlian Yang Chen Wang 

机构地区：[1]Key Laboratory for Biological Effects of Nanomaterials and Nanosafety(Chinese Academy of Sciences(CAS)),Key Laboratory of Standardization and Measurement for Nanotechnology(CAS),CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Nano Research》2023年第12期13294-13300,共7页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21721002,32101130,and 31971295);Financial support from the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)is also gratefully acknowledged.

摘　　要：We have determined the binding strengths between ribonucleotides of adenine(A),guanine(G),uracil(U),and cytosine(C)in homogeneous single-stranded ribonucleic acids(ssRNAs)and homo-decapeptides consisting of 20 common amino acids.We use a bead-based fluorescence assay for these measurements in which decapeptides are immobilized on the bead surface and ssRNAs are in solutions.The results provide a molecular basis for analyzing selectivity,specificity,and polymorphisms of amino-acid–ribonucleotide interactions.Comparative analyses of the distribution of the binding energies reveal unique binding strength patterns assignable to each pair of amino acid and ribonucleotide originating from the chemical structures.Pronounced favorable(such as Arg–G)and unfavorable(such as Met–U)binding interactions can be identified in selected groups of amino acid and ribonucleotide pairs that could provide basis to elucidate energetics of amino-acid–ribonucleotide interactions.Such interaction selectivity,specificity,and polymorphism manifest the contributions from RNA backbone,RNA bases,as well as main chain and side chain of the amino acids.Such characteristics in peptide–RNA interactions might be helpful for understanding the mechanism of protein–RNA specific recognition and the design of RNA nano-delivery systems based on peptides and their derivatives.

关 键 词：PEPTIDES single-stranded ribonucleic acid(ssRNA) binding affinity flow cytometry 

分 类 号：Q522[生物学—生物化学]