Single-cell manipulation by two-dimensional micropatterning  

作　　者：Xuehe Ma Haimei Zhang Shiyu Deng Qiushuo Sun Qingsong Hu Yuhang Pan Fen Hu Imshik Lee Fulin Xing Leiting Pan 

机构地区：[1]The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied Physics Nankai University,Tianjin 300071,P.R.China [2]State Key Laboratory of Medicinal Chemical Biology Frontiers Science Center for Cell Responses College of Life Sciences,Nankai University Tianjin 300071,P.R.China [3]Collaborative Innovation Center of Extreme Optics,Shanxi University Taiyuan,Shanxi 030006,P.R.China [4]Shenzhen Research Institute of Nankai University Shenzhen,Guangdong 518083,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第1期45-59,共15页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.12174208,32227802);National Key Research and Development Program of China(No.2022YFC3400600);Guangdong Major Project of Basic and Applied Basic Research(No.2020B0301030009);China Postdoctoral Science Foundation(No.2020 M680032);Fundamental Research Funds for the Central Universities(Nos.2122021337,2122021405);the 111 Project(No.B23045).

摘　　要：Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment mimicking.Here,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography micropatterning.Moreover,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication.

关 键 词：Two-dimensional micropatterning CYTOSKELETON cell migration extracellular matrix intercellular communication gene expression 

分 类 号：R318[医药卫生—生物医学工程]