Development of water-soluble AIE-based wash-free Aβprobes superior to commercial ThT  

作　　者：Ting-Ting Hou Ying-Hao Tang Zhen-Yu Zhang Ze-Jun Li Ya-Long Wang 

机构地区：[1]Key Laboratory of Biomedical Engineering of Hainan Province,School of Biomedical Engineering,Hainan University,Haikou,Hainan 570228,P.R.China [2]One Health Institute,Hainan University,Haikou,Hainan 570228,P.R.China [3]Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,Hubei 430074,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2023年第6期3-12,共10页创新光学健康科学杂志（英文）

基　　金：supported by the National Key R&D Program of China(2021ZD0201004);the National Natural Science Foundation of China(22165008,22077037,21474034,51673077 and 51603078);Hainan Provincial Natural Science Foundation of China(521RC506);the Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2020WNLOKF018)。

摘　　要：Alzheimer's disease(AD)is a typical neurodegenerative disease.β-amyloid(AβÞplaque is the most prominent pathological biomarker associated with the progression of AD.Conventional Aβprobes,including commercial probe ThT,usually suffer from tedious washing procedures.Herein,novel AIE-active Aβprobes with excellent water solubility,named DE-V1-PYC3 and DE-V1-PYOH,were developed for the detection and image of Aβwithout tedious washing procedures.Compared with commercial probe ThT,the AIE-active Aβprobes exhibited better sensitivity and a±nity to Aβaggregates.Moreover,for ThT,the washing procedures are essential to obtain high signal-to-noise ratio(SNR)images of Aβplaques in AD brain tissue slices.DE-V1-PYC3 and DE-V1-PYOH can label Aβplaques in AD brain tissue slices with high SNR even without tedious washing procedures.

关 键 词：Β-AMYLOID aggregation-induced emission fluorescence imaging 

分 类 号：R318[医药卫生—生物医学工程]