Clinical significance of platelet mononuclear cell aggregates in patients with sepsis and acute respiratory distress syndrome  

作　　者：Chong-Min Huang Juan-Juan Li Wei-Ke Wei 

机构地区：[1]Department of Intensive Medicine,The Second People's Hospital of Haining City,Haining 314419,Zhejiang Province,China [2]Department of Integrated Traditional Chinese and Western Medicine,Qingyang Hospital,Hangzhou 310023,Zhejiang Province,China

出　　处：《World Journal of Clinical Cases》2024年第5期966-972,共7页世界临床病例杂志

摘　　要：BACKGROUND The diagnosis of sepsis combined with acute respiratory distress syndrome(ARDS)has increased owing to the enhanced awareness among medical profes-sionals and the continuous development of modern medical technologies,while early diagnosis of ARDS still lacks specific biomarkers.One of the main patho-genic mechanisms of sepsis-associated ARDS involves the actions of various pathological injuries and inflammatory factors,such as platelet and white blood cells activation,leading to an increase of surface adhesion molecules.These adhesion molecules further form platelet-white blood cell aggregates,including platelet-mononuclear cell aggregates(PMAs).PMAs has been identified as one of the markers of platelet activation,here we hypothesize that PMAs might play a potential biomarker for the early diagnosis of this complication.METHODS We selected 72 hospitalized patients diagnosed with sepsis as the study population between March 2019 and March 2022.Among them,30 patients with sepsis and ARDS formed the study group,while 42 sepsis patients without ARDS comprised the control group.After diagnosis,venous blood samples were imme-diately collected from all patients.Flow cytometry was employed to analyze the expression of PMAs,platelet neutrophil aggregates(PNAs),and platelet aggregates(PLyAs)in the serum.Additionally,the Acute Physiology and Chronic Health Evaluation(APACHE)II score was calculated for each patient,and receiver operating characteristic curves were generated to assess diagnostic value.RESULTS The study found that the levels of PNAs and PLyAs in the serum of the study group were higher than those in the control group,but the difference was not statistically significant(P>0.05).However,the expression of PMAs in the serum of the study group was significantly upregulated(P<0.05)and positively correlated with the APACHE II score(r=0.671,P<0.05).When using PMAs as a diagnostic indicator,the area under the curve value was 0.957,indicating a high diagnostic value(P<0.05).Furthermore,the optimal cutoff value

关 键 词：SEPSIS Acute respiratory distress syndrome Platelet leukocyte aggregates Platelet mononuclear cell aggregates Biomarker 

分 类 号：R563.8[医药卫生—呼吸系统]