Long-term functional maintenance of primary hepatocytes in vitro using macroporous hydrogels engineered through liquid-liquid phase separation  

作　　者：Yang Sun Sheng Yin Jian Cui Zhongxia Wang Yueying Han Ding Ma Shuo Wang Junhua Wu Yi Cao Chunping Jiang Xiaosong Gu 

机构地区：[1]Jinan Microecological Biomedicine Shandong Laboratory,Jinan 250021,China [2]Collaborative Innovation Center of Advanced Microstructures,National Laboratory of Solid State Microstructure,Key Laboratory of Intelligent Optical Sensing and Manipulation,Ministry of Education,Department of Physics,Nanjing University,Nanjing 210093,China [3]Department of Hepatobiliary Surgery,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210093,China [4]National Institute of Healthcare Data Science at Nanjing University,Nanjing University,Nanjing 210093,China

出　　处：《Nano Research》2024年第3期1725-1736,共12页纳米研究（英文版）

基　　金：funded by the National Key R&D Program of China(No.2020YFA0908100);the Research Project of Jinan Microecological Biomedicine Shandong Laboratory(Nos.JNL2022004A,JNL2022019B);Shandong Provincial Laboratory Project(No.SYS202202).

摘　　要：Preserving the functionality of hepatocytes in vitro poses a significant challenge in liver tissue engineering and bioartificial liver,as these cells rapidly lose their metabolic and functional characteristics after isolation.Inspired by the macroporous structures found in native liver tissues,here we develop synthetic hydrogel scaffolds that closely mimic the liver’s structural organization through the phase separation between polyethylene glycol(PEG)and polysaccharides.Our hydrogels exhibit interconnected macroporous structures and appropriate mechanical properties,providing an optimal microenvironment conducive to hepatocyte adhesion and the formation of sizable aggregates.Compared to two-dimensional hepatocyte cultures,enhanced functionalities of hepatocytes cultured in our macroporous hydrogels were observed for 14 days,as evidenced by quantitative reverse-transcription–polymerase chain reactions(qRT-PCR),immunofluorescence,and enzyme linked immunosorbent assay(ELISA)analyses.Protein sequencing data further confirmed the establishment of cell–cell interactions among hepatocytes when cultured in our hydrogels.Notably,these hepatocytes maintained a protein expression lineage that closely resembled freshly isolated hepatocytes,particularly in the Notch and tumor necrosis factor(TNF)signaling pathways.These results suggest that the macroporous hydrogels are attractive scaffolds for liver tissue engineering.

关 键 词：liver tissue engineering phase separation HEPATOCYTES macroporous hydrogel 

分 类 号：O64[理学—物理化学]