Rapid and sensitive detection of Epstein-Barr virus antibodies in nasopharyngeal carcinoma by chemiluminescence strips based on iron-porphyrin single atom nanozyme  

作　　者：Daji Wang Jie Wang Dan Liu Jiuyang He Meiying Wang Haibing Huang Guohui Nie Hui Ding Xiyun Yan 

机构地区：[1]Nanozyme Synthesis Center,Shenzhen Institute of Synthetic Biology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [2]Center for Drug Evaluation,National Medical Products Administration,Beijing 100022,China [3]Shenzhen Key Laboratory of nanozymes and Translational Cancer Research,Department of Otolaryngology,and Shenzhen Institute of Translational Medicine,Shenzhen Second People’s Hospital/the First Affiliated Hospital of Shenzhen University Health Science Center,Shenzhen 518035,China [4]CAS Engineering Laboratory for Nanozyme,Key Laboratory of Protein and Peptide Pharmaceutical,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [5]Department of Rheumatology and Immunology,Shenzhen Second People’s Hospital,The First Affiliated Hospital of Shenzhen University,Shenzhen 518055,China

出　　处：《Nano Research》2024年第3期1827-1836,共10页纳米研究（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2019YFA0709204);the National Natural Science Foundation of China(No.32200744);the Natural Science Foundation of Guangdong Province(Nos.2021A1515110028 and 2022A1515220147);the Science and Technology Program for Basic Research in Shenzhen(Nos.JCYJ20210324103015039,JCYJ20190809095811254,and JCYJ20200109140412476);the National Natural Science Foundation of China(Nos.81930050 and 22121003).

摘　　要：The correlation between Epstein-Barr virus(EBV)infection and nasopharyngeal carcinoma(NPC)risk has been extensively researched.The continual monitoring of EBV-IgAs provides a promising approach of NPC screening in its early stage.In this study,we successfully synthesized a single-atom nanozyme(SANzyme)through the application of iron-porphyrin based metal organic framework(MOF-FeP).The MOF-FeP possesses precisely-defined electronic and geometric structures that accurately mimic highly-evolved catalytic site of natural peroxidase.The peroxidase-like activity of MOF-FeP enables it to catalyze the chemiluminescence of luminol substrate.By integrating MOF-FeP into a traditional strip,we created a rapid and highly-sensitive evaluation tool for detecting EBV-IgAs.Importantly,the MOF-FeP strip enables the simultaneous detection of three EBV-IgAs,greatly improving the accuracy of EBV-associated NPC screening.The sensitivities of the MOF-FeP strip(75.56%–93.30%)surpass those of current enzyme-linked immunosorbent assay(ELISA)methods(64.44%–82.22%).This test takes only 16 min to perform as opposed to the customary 1–2 h required for standard ELISA.Additionally,the MOF-FeP strip is suitable for whole blood samples,thereby significantly simplifying the sample preparation and detection process.In conclusion,the MOF-FeP strip combines the simplicity of traditional strip with the high catalytic activity of SANzyme.Our innovative MOF-FeP strip offers a new point-of-care strategy for EBV-IgAs detection,which is expected to markedly facilitate early screening for EBV-associated diseases.

关 键 词：IRON-PORPHYRIN single-atom nanozyme(SANzyme) chemiluminescence strips Epstein-Barr virus(EBV) nasopharyngeal carcinoma(NPC) antibody detection 

分 类 号：R739.63[医药卫生—肿瘤]