一次离心法制备兔贫白细胞富血小板血浆的离心条件研究  

Study of centrifuge conditions for preparing rabbit leukocyte-poor platelet-rich plasma by single centrifugation

作　　者：张克凡 张晨晨[2] 任强[1] 王德宁 孙力 王鑫 王耀敏 石辉[1] ZHANG Kefan;ZHANG Chenchen;REN Qiang;WANG Dening;SUN Li;WANG Xin;WANG Yaomin;SHI Hui(Department of Orthopedics and Sports Medicine,Binzhou Medical University Hospital,Binzhou Shandong,256603,P.R.China;Department of Operating Room,Binzhou Medical University Hospital,Binzhou Shandong,256603,P.R.China;Department of Orthopedics,Nanyang Central Hospital,Nanyang Henan,473009,P.R.China)

机构地区：[1]滨州医学院附属医院骨关节与运动医学科,山东滨州256603 [2]滨州医学院附属医院手术室,山东滨州256603 [3]南阳市中心医院骨科,河南南阳473009

出　　处：《中国修复重建外科杂志》2024年第2期183-188,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：山东省省级临床重点专科学科建设项目(SLCZDZK-0302);山东省医药卫生科技发展计划项目(2017WS040);2020年产学合作协同育人项目(202002204024);滨州医学院徐荣祥再生医学发展计划项目(BY2019XRX04)。

摘　　要：目的探究使用一次离心法制备兔贫白细胞富血小板血浆(leukocyte-poor platelet-rich plasma,LPPRP)的最佳离心条件。方法取16只3~4月龄健康新西兰兔,于兔耳背中央动脉采集15 mL抗凝血,分别于1、2个月后重复取血步骤。将所取血液标本于离心半径16.7 cm分别以转速1200、1300、1400、1500 r/min(对应离心力分别为269×g、315×g、365×g、420×g)下离心2、3、4、5 min,共计16组。各组离心后采集分离平面1.5 mL以上的血浆,计算并比较不同离心条件下各组LP-PRP的体积、血小板富集系数及血小板回收率。结果所有离心条件下获得的LP-PRP体积范围为1.8~7.6 mL。相同离心转速时,随离心时间延长,LP-PRP体积显著增大,同时LP-PRP血小板富集系数呈降低趋势。离心2 min时,1200、1300 r/min转速下LP-PRP体积<2.0 mL,其余条件下获得的LP-PRP体积均≥2.0 mL;离心4、5 min时,各转速下获得的LP-PRP体积均≥4.0 mL。1200 r/min转速下各离心时间组和1300 r/min转速下离心2、3 min组LP-PRP血小板富集系数均>2.0,其中1200 r/min转速下离心2 min能够获得最高的LP-PRP血小板富集系数。1200 r/min转速下离心4、5 min与1400 r/min转速下离心5 min获得的LP-PRP血小板回收率均>60%,1200 r/min转速下离心4、5 min组间比较差异无统计学意义(P>0.05)。结论采用一次离心法制备兔LP-PRP时,采集15 mL血液以离心半径16.7 cm、1200 r/min转速下离心4 min即可制备体积>2.0 mL、血小板富集系数>2.0且血小板回收率>60%的LP-PRP,在最短时间达到LP-PRP的最佳作用参数。Objective To explore the best centrifuge condition for preparing rabbit leukocyte-poor platelet-rich plasma(LP-PRP)by using single centrifugation method.Methods Sixteen healthy New Zealand rabbits,aged 3-4 months,were utilized in the investigation.A total of 15 mL anticoagulated blood was extracted from the central ear artery of each rabbit,with a repeat of the blood collection procedure after 1 and 2 months.The obtained blood specimens were individually subjected to centrifugation at a radius of 16.7 cm and speeds of 1200,1300,1400,and 1500 r/min(equivalent to centrifugal forces of 269×g,315×g,365×g,and 420×g)for durations of 2,3,4,and 5 minutes,resulting in a total of 16 groups.Following centrifugation,collect plasma from each group to a distance of 1.5 mL from the separation plane.The volumes,platelet enrichment coefficient,and platelet recovery rates of LP-PRP in each group,under varying centrifugation conditions,were methodically computed and subsequently compared.Results The volume of LP-PRP obtained under all centrifugation conditions ranged from 1.8 to 7.6 mL.At a consistent centrifugal speed,an extension of centrifugation time leaded to a significant increase in the volume of LP-PRP,accompanied by a declining trend in the platelet enrichment coefficient of LP-PRP.When centrifuged for 2 minutes,the volume of LP-PRP at speeds of 1200 and 1300 r/min was less than 2.0 mL,while the volume of LP-PRP obtained under other conditions was more than 2.0 mL.When centrifuged for 4 and 5 minutes,the volume of LP-PRP obtained at each speed was more than 4 mL.LP-PRP with a platelet enrichment coefficient more than 2.0 could be prepared by centrifuging at 1200 r/min for each time group and 1300 r/min for 2 and 3 minutes,and the highest LP-PRP platelet enrichment coefficient could be obtained by centrifugation for 2 minutes at a speed of 1200 r/min.The platelet recovery rates of LP-PRP obtained by centrifugation at 1200 r/min for 4 and 5 minutes,as well as centrifugation at 1400 r/min for 5 minutes,were both greater t

关 键 词：一次离心法 贫白细胞富血小板血浆 离心条件 兔 

分 类 号：R-33[医药卫生]