改良Videman法构建兔膝骨关节炎模型的探索  

Exploration of constructing a rabbit knee osteoarthritis model using the modified Videman method

作　　者：罗娆珊 唐青梅 龚广 余立志 罗三彤[1] 梁伟明 LUO Raoshan;TANG Qingmei;GONG Guang;YU Lizhi;LUO Santong;LIANG Weiming(The First Affiliated Hospital of Guangxi University of Science and Technology,Guangxi,Liuzhou 545000,China)

机构地区：[1]广西科技大学第一附属医院,广西柳州545000

出　　处：《中国医药科学》2024年第1期25-28,共4页China Medicine And Pharmacy

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20211376);广西科技计划项目(广西科技基地和人才专项)(桂科AD19245017);广西科技大学博士基金项目(校科博20Z13)。

摘　　要：目的建立操作简便、稳定、高效,便于治疗和疗效观察的兔膝骨关节炎(KOA)模型。方法选取12只健康雄性新西兰兔,按照随机数表法分为对照组、模型组,每组各6只。使用医用夹板将兔右膝关节伸直位固定,每次固定60 min,每天2次,持续4周进行造模。采用Lequesne MG评分法对兔子右膝关节的运动能力进行评估,观察并记录兔子右膝关节的大体形态,番红O染色判断软骨损伤情况,采用改良Mankin’s关节软骨病理评分法对两组兔子进行比较分析。结果模型组Lequesne MG评分综合得分为[2.00(0.00,8.00)]分,高于对照组,差异有统计学意义(P<0.05)。观察膝关节大体形态结果,与对照组比较模型组膝关节肿大,关节腔隙变窄,软骨部分磨损,表面质地粗糙。观察番红O染色结果,与对照组比较模型组软骨组织结构紊乱,基质染色减少,潮线不完整,软骨表层不平整,软骨细胞形态呈多样性,排列不规则。观察改良Mankin’s关节软骨病理评分结果,模型组综合得分为[3.00(0.00,7.50)]分,高于对照组,差异有统计学意义(P<0.05)。结论改良Videman法构建兔KOA模型,具有操作简便、固定牢固、成功率高等优点,有利于KOA发生发展机制的研究。Objective To establish a rabbit knee osteoarthritis(KOA)model that is easy to operate,stable,efficient,and convenient for treatment and efficacy observation.Methods A total of 12 healthy male New Zealand rabbits were divided into a control group and a model group using the random number table method,with 6 rabbits in each group.The medical splint was used to fix the right knee joint of the rabbit in an extended position,with each fixation lasting for 60 minutes,twice a day,and lasting for 4 weeks for modeling.The Lequesne MG scoring method was used to assess the motor ability of the rabbit’s right knee joint,the general morphology of the rabbit’s right knee joint was observed and recorded,safranine O staining was used to determine cartilage damage,and the modified Mankin’s joint cartilage pathology scoring method was used to compare and analyze the two groups of rabbits.Results The comprehensive Lequesne MG score of the model group was(2.00[0.00,8.00])points,which was higher than tha t of the control group,with statistically significant difference(P<0.05).The general morphology of the knee joint showed that compared with the control group,the model group had swollen knee joints,narrowed joint cavities,worn cartilage,and rough surface texture.The results of safranine O staining showed that compared with the control group,the model group had a disordered cartilage tissue structure,reduced matrix staining,incomplete tidal line,uneven surface of cartilage,and a diverse and irregular arrangement of chondrocytes.In terms of the modified Mankin’s joint cartilage pathology score,the comprehensive score of the model group was(3.00[0.00,7.50])points,higher than that of the control group,with statistically significant difference(P<0.05).Conclusion The modified Videman method for constructing a rabbit KOA model has the advantages of simple operation,firm fixation,and high success rate,which is conducive to the study of the mechanism of KOA occurrence and development.

关 键 词：膝骨关节炎 动物模型 改良Videman法 评分 

分 类 号：R-332[医药卫生]