石蒜碱通过降解SCAP蛋白抑制口腔鳞癌细胞增殖与侵袭的实验研究  

Lycorine suppressed oral squamous cell carcinoma cell proliferation and invasion via scap protein degradation: an experimental study

作　　者：李华盛 周迪 韩楠男 严明[1] 阮敏[1] LI Hua-sheng;ZHOU Di;HAN Nan-nan;YAN Ming;RUAN Min(Department of Oromaxillofacial Head and Neck Oncology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology.Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院、口腔颌面-头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《中国口腔颌面外科杂志》2024年第1期29-35,共7页China Journal of Oral and Maxillofacial Surgery

摘　　要：目的:探讨天然植物提取物石蒜碱对口腔鳞癌细胞的作用及相关机制。方法:应用平板克隆形成实验和流式细胞术检测石蒜碱对口腔鳞癌细胞体外增殖的作用,采用划痕实验和Transwell实验检测石蒜碱对口腔鳞癌细胞侵袭的作用,应用Western免疫印迹和免疫荧光验证石蒜碱对口腔鳞癌细胞SCAP蛋白的抑制作用。构建裸鼠荷瘤模型检测石蒜碱的体内抗口腔鳞癌效果。采用Graphpad Prism软件包进行统计学分析。结果:平板克隆形成和流式细胞实验显示,石蒜碱对口腔鳞癌细胞的体外增殖具有明显的抑制作用。划痕实验和Transwell结果显示,石蒜碱对口腔鳞癌细胞的体外迁移、侵袭也具有明显的抑制作用。Western免疫印迹和免疫荧光结果显示,石蒜碱能够降解口腔鳞癌细胞的SCAP蛋白,影响癌细胞的胆固醇代谢。动物实验结果进一步表明,石蒜碱在体内也具有良好的抗癌活性,能显著抑制口腔鳞癌细胞的生长,降低癌细胞内SCAP蛋白水平。结论:石蒜碱能够通过降解SCAP蛋白,抑制口腔鳞癌细胞增殖与侵袭,有望成为治疗口腔鳞癌的低毒高效天然植物提取药物。PURPOSE:To investigate the effects of Lycorine,a natural plant extract,on oral squamous cell carcinoma(OSCC)cells and its underlying mechanisms.METHODS:Plate cloning assay and flow cytometry were used to evaluate the inhibitory effect of Lycorine on the proliferation of OSCC in vitro.Scratch assay and Transwell assay were conducted to assess the impact of Lycorine on the invasion of OSCC.Western blot and immunofluorescence were performed to confirm the inhibitory effect of Lycorine on SCAP protein in OSCC.A nude mouse xenograft model was established to evaluate the in vivo anticancer effect of Lycorine against oral squamous cell carcinoma.Graphpad Prism software package was used for statistical analysis.RESULTS:Plate cloning assay and flow cytometry showed that Lycorine significantly inhibited the proliferation of OSCC cells in vitro.Scratch assay and Transwell assay revealed a significant inhibitory effect of Lycorine on the migration and invasion of OSCC cells.Western blot and immunofluorescence results demonstrated that Lycorine could degrade SCAP protein in OSCC cells,affecting cholesterol metabolism in cancer cells.Animal experiment results further indicated that Lycorine exhibited potent anticancer activity in vivo,significantly suppressing the growth of OSCC cells and reducing the levels of SCAP protein within cancer cells.CONCLUSIONS:Lycorine can suppress the proliferation and invasion of OSCC cells by degrading SCAP protein,making it a potential low-toxicity and highly effective natural plant extract for the prevention and treatment of OSCC.

关 键 词：石蒜碱 口腔鳞癌 甾醇调节因子结合蛋白裂解激活蛋白 增殖 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]