特立帕肽对骨质疏松大鼠正畸牙移动中牙根吸收影响的mirco-CT研究  

Effect of Teriparatide on Root Resorption during Orthodontic Tooth Movement in Ovariectomized Rats by Micro-CT

作　　者：宿晨曦 张程 何姝姝[1] 邹淑娟[1] SU Chenxi;ZHANG Cheng;HE Shushu;ZOU Shujuan(State Key Laboratory of Oral Disease,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Department of Orthodontics,Tianjin Stomatological Hospital,School of Medi-cine,Nankai University,Tianjin 300041,China;Tianjin Key Laboratory of Oral and Maxillofacial Func-tion Reconstruction,Tianjin 300041,China.)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床研究中心,四川大学华西口腔医院,四川成都610041 [2]天津市口腔医院正畸科,南开大学医学院,天津300041 [3]天津市口腔功能重建重点实验室,天津300041

出　　处：《口腔医学研究》2024年第2期136-141,共6页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81470777);天津市医学重点学科(专科)建设项目资助(编号:ZDPY-05);四川省科技计划资助项目(编号:2022NSFSC1376)。

摘　　要：目的:使用mirco-CT探究特立帕肽对骨质疏松大鼠正畸牙移动过程中牙根吸收的影响。方法:将72只8周龄的雌性Wistar大鼠均分为去势组、特立帕肽组和对照组,对去势组及特立帕肽组行去卵巢手术并对对照组行假手术处理。12周后,对大鼠左上第一磨牙施加50 g牙移动力;每日给予特立帕肽组大鼠特立帕肽溶液皮下注射,去势组及对照组给予等量的PBS溶液。牙移动第0、7、14、21天处死大鼠、取材并行micro-CT扫描分析。结果:各组从7 d起可开始观察到牙根吸收陷窝,且陷窝体积随时间延长逐渐增大。去势组大鼠的牙根吸收与对照组相比较为严重,特立帕肽治疗可以显著降低骨质疏松大鼠牙移动21 d时的牙根吸收。结论:骨质疏松状态可加重大鼠牙移动过程中的牙根吸收,间歇性应用特立帕肽可以减少骨质疏松大鼠正畸牙移动中的牙根吸收。Objective:To investigate the effect of teriparatide on root resorption during orthodontic tooth movement in osteoporotic rats using micro-CT.Methods:Seventy-two female Wistar rats(8-week-old)were randomly divided into 3 groups as follows:ovariectomized group,teriparatide group(ovariectomized rats treated by teriparatide),and the control group(sham-operated).After twelve weeks,50g of force was placed to move the first molar on the left side by using a closed-coil spring.The teriparatide group was given subcutaneous injection of teriparatide daily,while the ovariectomized group and control group were given equal amounts of PBS solution.Rats were sacrificed on days 0,7,14,and 21,and samples were collected and subjected to micro-CT scanning.Results:The root resorption lacunae can be observed in all groups from day 7,and the volume of the lacunae increased gradually with time.The root resorption of rats in ovariectomized group was more serious than that in control group.Teriparatide treatment could significantly reduce the root resorption of ovariectomized rats on day 21.Conclusion:Osteoporosis can aggravate root resorption during tooth movement in rats,and intermittent teriparatide administration can reduce orthodontically induced root resorption in osteoporotic rats.

关 键 词：骨质疏松 正畸牙移动 特立帕肽 牙根吸收 

分 类 号：R783.5[医药卫生—口腔医学] R580[医药卫生—临床医学]