乌拉尔甘草不同组织可培养内生菌分离筛选及产β-葡萄糖苷酶菌株初筛  

Isolation and screening of cultivable endophytes in sifferent organs of Glycyrrhiza uralensis Fisch and preliminary screening ofβ-glucosidase producing strains

作　　者：孔晓双 魏然[2] 董应宏 侯敏[3] 买尔哈巴·艾合买提[3] 侯新强[3] 杨文琦[3] 崔卫东[3] KONG Xiaoshuang;WEI Ran;DONG Yinghong;HOU Min;Maierhaba Aihemaiti;HOU Xinqiang;YANG Wenqi;CUI Weidong(College of Life Science and Technology,Xinjiang University,Urumqi 830052,China;College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Xinjiang Special Environmental Microbiology Laboratory,Urumqi 830091,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830052 [2]新疆农业大学农学院,乌鲁木齐830052 [3]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2024年第1期199-208,共10页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发任务专项项目(2022B02042);新疆维吾尔自治区重点研发计划项目(2022B02056-2)。

摘　　要：【目的】研究新疆阿勒泰地区乌拉尔甘草不同组织可培养内生菌的资源状况以及选育高产β-葡萄糖苷酶菌株。【方法】以乌拉尔甘草为材料,运用组织匀浆法与16S rDNA和ITS-rDNA分子生物学方法相结合方式,对其主根、须根、茎、叶中的内生菌进行分离、纯化和鉴定;采用七叶苷培养基和β-葡萄糖苷酶酶活力测定筛选产β-葡萄糖苷酶菌株。【结果】从甘草中分离获得内生菌共48株,其中内生细菌33株,内生真菌15株。在主根和茎部位分离内生细菌数量最多,内生真菌在主根中定殖的数量最多。33株甘草内生细菌隶属于8个属,芽孢杆菌属菌株分离获得最多,且甘草主根部位的内生细菌分离种类最多,在甘草不同组织中多样性表现为主根>须根=茎=叶。15株甘草内生真菌隶属于7个属,枝孢属是甘草内生真菌的优势属,内生真菌在不同组织中多样性表现为主根>茎>叶>须根。共有12株具有产β-葡萄糖苷酶的能力,其中分离自主根部位的菌株最多,占比41.7%。【结论】甘草主根组织的可培养内生菌的多样性相较其他组织丰富,且主根部位产β-葡萄糖苷酶菌株较多,是选育产酶菌株的优势部位。【Objective】To study the resource status of culturable endophytes from different parts of Glycyrrhiza uralensis Fisch Altay region of Xinjiang and to screen high-yieldβ-glucosidase strains.【Methods】Using Glycyrrhiza uralensis Fisch as the material,the endophytic bacteria in its taproot,fibrous root,stem and leaf were isolated,purified and identified by means of tissue homogenization combined with 16S rDNA and ITS-rDNA molecular biology methods.Theβ-glucosidase producing strains were screened by aescin medium andβ-glucosidase activity determination.【Results】The results showed that 48 endophytes,33 endophytic bacteria and 15 endophytic fungi were isolated from Glycyrrhiza uralensis Fisch.The number of endophytic bacteria isolated from taproots and stems was the largest,and that of endophytic fungi colonized in taproots was the largest.The 33 endophytic bacteria from Glycyrrhiza uralensis Fisch belonged to 8 genera,and the most Bacillus were isolated,and the endophytic bacteria in the taproot of Glycyrrhiza uralensis Fisch were the most widely isolated species.The diversity in different parts was as follows:taproot>fibrous root=stem=leaf.15 endophytic fungi from Glycyrrhiza uralensis Fisch belonged to 7 genera,Cladosporium was the dominant genus of endophytic fungi.The diversity of endophytic fungi in different parts was as follows:taproot>stem>leaf>fibrous root.A total of 12 strains had the ability to produceβ-glucosidase,among which the most strains isolated from taproots,accounted for 41.7%.【Conclusion】The diversity of taproot organ of cultivable endophytes from Glycyrrhiza uralensis Fisch is rich and there are moreβ-glucosidase producing strains,which are the dominant part for breeding enzyme-producing strains.

关 键 词：乌拉尔甘草 可培养内生菌 筛选 Β-葡萄糖苷酶 

分 类 号：S188[农业科学—农业基础科学] S812